54 THE MICROSCOPE. 



Methyl anilin violet, and iodine, malachite or methyl green 

 combined give beautiful results. Aqueous solutions were first 

 worked, but now an alcoholic mixture is used in the following way: 

 Make a saturated solution of violet in strong alcohol and filter. Do 

 the same with the green, leaving some granules in excess at the bot- 

 tom of the vessel; this solution is a greenish-blue. Filter and add 

 the violet solution to it, drop by drop, till the mixture is the^ color of 

 Prussian blue, which soon occurs. If a large quantity be made for 

 future use, add a little strong carbolic acid solution about three 

 drops to the ounce of the solution. Filter about twenty drops into 

 a watch glass and dilute with spirit and immerse sections for five' to 

 ten minutes; wash off excess in ordinary water, or better drain off 

 on filter paper, and set in saturated solution of acetate of potash. 

 This gives good preparation for several weeks, but the dyes may be 

 fixed by placing the sections for three to five minutes in a mixture 

 of equal parts of a pure saturated aqueous solution of tannin, to 

 which a little carbolic acid has been added, and distilled water. 

 Then wash in water and transfer, for the same time, to a mixture of 

 rather more than one part of tartar emetic and rest distilled water; 

 wash and put in strong methylated spirit for five to ten minutes; 

 draw off excess of spirit on filter paper, and put for five minutes in 

 oil of cajeput, cloves, juniper, aniseed or turpentine, and mount after 

 drawing off excess of oil — in Canada balsam or dammar. The tan- 

 nin and antimony solutions should be filtered into a watch glass 

 before using, as also should be the dyes. If this process be success- 

 fully carried out, very striking results are obtained. Connective 

 and elastic tissue and cell protoplasm are stained violet; nuclei, 

 nucleoli, young cells and leucocytes are greenish-blue. The nuclei 

 of unstriped muscle-fibres and the nuclei of the sarcolemma of 

 striped fibres are blue, and blood is rusty brown. The young cells 

 of tumors, especially cancer and sarcoma, are greenish-blue. Sec- 

 tions of lymphatic glands are very pretty, the leucocytes being blue 

 and the rest violet; and, if the tumor cells be present, they are 

 greenish-blue and readily made out. — British Medical Journal. 



Minute Pathology. — Fehleisen has cultivated micrococci 

 taken from the lymphatic vessels and subcutaneous connective 

 tissue of patients suffering from erysipelas. Inoculations were per- 

 formed on rabbits and on man with success. Prompted by the 



