u 



THE MICROSCOPE. 135 



with an animalcule furnishes another interesting illustration of the 

 approximation of the lowest animal and vegetable organism. — 

 Acad. Nat. Sc. Philadelphia. 



To Stain Bacillus Tuberculosis. — Method devised by 

 Ehrlich, modified by Gibbes : 

 No. 1— 



(a) Magenta crystals, - - 2 grammes. 



(b) Pure aniline (oil), - - 3 " 



(c) Alcohol, sp. gr. 880, - 20 



(d) Distilled water, - - 20 

 Mix (b) and (c) and add to pulverized (a) slowly while stirring 



and add (d) in same way. Bottle without filtering. 

 No. 2 — 



Nitric acid, -------- 1 part. 



Distilled water, 2 parts. 



No. 3 — 



Saturated alcoholic solution of 



methyline blue, ----- 1 part. 



Distilled water, ------ 4 parts. 



Thoroughly dry a thin film of sputa on cover glass ; immerse 

 (or float on) in No. 1 at 104° Fah. 20 minutes ; No. 2 long enough 

 to remove stain from everything except the bacilli (1 — 10 min); 

 thoroughly wash in distilled water (30 min.); No. 3, one hour ; wash 

 in water. When perfectly dry mount dry or in balsam. A good % 

 in. objective should show the organisms — the bacilli red, other 

 material blue. 



Histology of Insect Wing-Muscles. — The memoir of G. V. 

 Ciaccio, to appear shortly in the Memorie dell' accademia di 

 Bologna, may be thus summarized: In most insects the. wing- 

 muscles may be decomposed into fibrillar (in others, into striated 

 fibres: Sphinx, Libellula, etc.). In the former case the fibrillar are 

 united into bundles of various sizes by a cementing substance, in 

 which the nuclei lie either both in the interior and upon the surface 

 of the bundle (Hydrophilus, Dytiscus), or upon the surface only 

 (flies). The bundles are held together by trachea?, and sometimes 

 also by fat-cells. In the cement are further always found distinct 

 particles (Aubert's masse grameleuse inter fibrillaire), which do not 

 occur in the other muscles. The fibres are composed of fibrillar, 



