150 Tue Microscope. 
Rub thoroughly in a mortar and add slowly 300 c. c. of dis- 
tilled water, mix carefully and filter. To the filtrate add 20c.c. 
of absolute alcohol. Preserve in a stoppered bottle. It should 
be kept in a cool place, and the older it becomes, the more 
excellent it is. 
An alcoholic solution is made as follows: 
Make three saturated solutions. 
a. Calcic chloride in 70 per cent. alcohol. 
6. Pulverized alum in 70 per cent. alcohol. 
ce. Hematoxylin in absolute alcohol. 
Mix one part of a. in eight parts of 6. and to the mixture 
add c. drop by drop until there is a deep purple color to the 
solution... Use only a small quantity of absolute alcohol for ce. 
as the logwood is very soluble. The color deepens and improves 
with age. <A few drops of this solution added to a watch-glass 
full of water will make it of the proper strength for use. 
Alcoholic solutions of logwood do not require to be filtered 
so frequently as the aqueous solutions. 
Sections should be left in the staining until they have 
acquired the proper depth of color. This is ascertained by 
taking out a section now and then and examining it. If not 
stained deep enough they should be submitted longer to the 
action of the coloring solution. If stained just deep enough 
they should be, washed in distilled water and then placed in 
alcohol. If stained too deeply they should be transferred to a 
solution of acetic acid for a short time. The strength of the 
acid solution will depend upon the amount of color it is desired 
to remove from the specimen; varying then from a five per 
cent. to a fifty per cent. solution. The action of the acid should 
be carefully watched and when the color has been sufficiently 
reduced the sections should be transferred at once to alcohol. 
CARMINE STAINING. 
Beale’s carmine staining. 
Carmine, - - - - - = = = - OQ gtaig# 
Strong liquor ammonia, - - - - dram. 
Price’s glycerine, - - - - - - 2 ounces. 
Distilled water, - - - - - - - 2ounces. 
Aleohol,; - <= = - <=)= == - 93 @umee 
