Tue Microscope. 153 
PICROCARMINE AND HA MATOXYLIN. 
Stain the sections shghtly in picrocarmine, wash them well 
in water, and transfer them to a weak solution of the logwood. 
This is useful for studying the development of bone, for the 
mesentery, etc. 
IODINE-GREEN. 
Make a saturated solution in distilled water and dilute to 
a one per cent. solution when required for use. It stains rap- 
idly, the staining cannot be removed by washing, and it gives 
different tints to the different tissues. It is really then a mul- 
tiple stain. 
PICROCARMINE AND IODINE-GREEN. 
Stain the sections in picrocarmine and wash them with 
slightly acidulated water. Then place in a one per cent. solu- 
tion of the iodine-green, watching them that they do not be- 
come stained too deeply. The sections should not be left long 
in the alcohol when placed there prior to mounting, for it will 
remove more or less of the green color. Bone, intestine, gland- 
tissue, cartilage, etc., are beautifully stained, and the parts 
finely differentiated, by this method. 
SULPHINDIGOTATE OF SODA AND CARMINE. 
The sections are stained in carmine as though they were to 
be stained in that reagent only. After fixing the carmine stain- 
ing in the dilute acid the sections are transferred to a large 
quantity of alcohol, where they are allowed to remain for two 
hours or more. They are then transferred to the solution of the 
soda in alcohol. Two or three drops of the saturated aqueous 
solution of the soda should be added to each ounce of the alco- 
hol, and the sections allowed to remain in it for four or five 
hours. 
There are a large number of mixtures recommended for 
double, treble, and even quadruple stainings. Those who are 
interested in investigating the subject of stains will find a 
variety of solutions to experiment with. 
(‘“‘Staining”’ continued in the next number.) 
