NOTES AND QUERIES. 163 
have yet tried for dissolving the imbedding mass. ‘The sections 
are usually freed from imbedding mass within half an hour, though 
the slide may remain in any of the solvents mentioned for two or 
three days, or perhaps indefinitely, without loosening the sections. 
When ihe slide is removed from the naphtha, the sections are 
washed with 95 p.c. alcohol by means of a medicine-dropper, or by 
immersing the slide in alcohol. If the sections are to be stained 
in Kleinenberg’s hematoxylin or in any other stain containing 50 
p.c. or more alcohol, the slide is transferred directly from the 
alcohol used for rinsing to the staining agent, otherwise it should 
be first transferred to 50 p.c. alcohol and from that to the staining 
agent. Whenever the sections are sufficiently stained they may be 
mounted in any desired mounting medium. In case Canada 
balsam is to be used, the slide must be immersed in alcohol to 
wash away the stain, and finally in 95 p.c. alcohol to completely 
anhydrate the sections. They are cleared with a mixture of 
carbolic acid 1 part, turpentine 4 parts. ‘The balsam to be used is 
prepared by mixing 25 grams of pure Canada balsam with acc. of 
chloroform and ezcc. of clove oil. The latter very soon removes 
any cloudiness that may have appeared in the collodion film.”— 
American Naturalist. 
NOTES AND QUERIES. 
AS we wish to relieve ourselves of all purely 
business transactions in connection with the Journal, 
Subscribers are kindly requested to pay the amount 
of their subscriptions to Messrs. Brook & Chrystal, 
11, Market-street, Manchester. 
ALL matter intended for publication must be sent before the 12th 
of each month to the Editor, Mr. George E. Davis, Belmont, 
Thorncliffe, nr. Sheffield. 
Errata.—Last month the printers took it into their heads to go 
to press without sending us a revise, several errors therefore exist 
in the May number :— 
Page 133, 4 lines from the bottom should read :— 
“in this case (12 x 1)* =169 and 120+169="71.” 
With reference to the article on the ‘‘ Cholera Germ,” on page 
_ 126, see the paragraph in our present ‘ Notes and Queries.” 
