270 THE MICROSCOPICAL NEWS. 
mixture ensured by frequent agitation.” The deep blue fluid thus 
prepared must be added to one ounce of glycerine, one ounce of 
methylated spirit and four ounces of water, as in the Prussian 
Blue fluid. Dr. Beale considers these proportions unnecessarily 
large, and gives the following recipe, which, however, I have not 
myself found to answer well, the injection, “especially under high 
powers, being too faint. 
Ferridcyanide of Potassium... ... 10 grains, 
Sulphate of Irom...) |)... <4.) 1) Sogn 
Water sect ascw Sheth tae! ee eee 
Glycerin’) ics). Gee ipises “nen eee 
PICONOl ss reek aa. -..  Eodrachuil 
The advantage of this injection ¢ over Prussian Blue is that the 
colour does not fade in course of time. 
BRUCKE’S SOLUBLE PRUSSIAN BLUE. 
A. Ferrocyanide of Potassium, 217 grammes dissolved in one litre 
of distilled water. 
B. Perchloride of Iron, 10 grammes dissolved in two litres of dis- 
tilled water. 
C. A cold saturated solution of Sulphate of Soda. 
Mix one part of A with one part of C (a), and mix one part of 
B with one part of C (8). Add £ to a, and allow the mixture to 
stand about three hours (longer if necessary), collect the deposit 
on a filter. Wash the deposit three or four times a day for a 
week by pouring over it small quantities of distilled water. The 
washing must be discontinued as soon as the water which runs 
through is quite blue; and the powder thus prepared must be dis- 
solved in distilled water and mixed with sufficient gelatine to form 
a firm jelly. 
DR. BEALE’S ACID CARMINE FLUID. 
Dr. Beale says, “ After trying a great many different combina- 
tions, I arrived at the following, which answers the purpose 
exceedingly well :— 
Carmine. )3..9 5. 5 grains, 
Glycerine, with 8 of 10 "drops of oy on 
Acetic or Cee Acid.. 
Glyceniner 2) tk. JS Aci SSO Saas 
Allcohol soils, ie! Me lee ee 
Water ere gels aps) Vedi ain 5 
Ammonia... ... a few drops. 
Mix the Carmine with a few “drops of water, and, when well in- 
corporated, add about five drops of liquor ammonia. To this 
dark red solution, about half an ounce of the glycerine is to be 
added, and the whole well shaken in a bottle. Next, very 
gradually pour in the acid glycerine, frequently shaking the bottle 
