9 
With  progressively  diminishing  quantities  of 
creosote,  we  find  a  pretty  good  development 
in  the  culture  at  0,01  °/o,  and  this  develop¬ 
ment  becomes  still  more  acentuated  when 
the  percentage  of  creosote  is  yet  smaller. 
Agar  with  sugar  (2  °/o). 
The  development  is  marked  and  the  vel¬ 
vety  aspect  is  striking.  The  culture  arises  se¬ 
veral  millimeters  from  the  surface  of  the 
medium  and  grows  thick  and  resistant.  The 
folds  are  high,  broad  and  numerous.  To  a 
high  fold  succeeds  a  profound  depression. 
Intermediate  folds  and  a  border  are  wan¬ 
ting.  This  is  a  good  medium  for  the  artifici¬ 
al  culture  of  the  O.  brasiliense. 
Glycerine— gelatine. 
It  is  liquefied  within  30  days.  A  thick, 
yellowish  pellicle  on  the  surface. 
Simple  broth. 
Formation  of  flakes.  A  thick,  dark-gray 
pellicle  rapidly  forms.  After  several  months 
there  is  a  brownish-white  deposit  and  a 
clear  liquid  over  it.  No  clouding  is  obcrved. 
This  medium  contains  many  yeast  cells  and 
mycelium  forms  of  the  fungus. 
Glycerine  broth. 
In  our  studies  we  preferred  this  me¬ 
dium  for  obtaining  large  quantities  of  the 
fungus.  Balloons  of  3  to  4  liters,  sown  with 
O.  brasiliense ,  show,  after  6  or  8  days,  the 
beginning  formation  of  the  dark-brown  pelli¬ 
cle.  Once  formed,  it  spreads  rapidly  all  over 
the  surface  of  the  fluid.  Little  by  little  it  at¬ 
tains  the  thickness  of  several  millimeters  and 
becomes  tomentous.  After  15  or  20  days 
more  or  less  dense  flakes  begin  to  sink  from 
the  lower  face  of  the  large  floating  pellicle 
to  the  interior  of  the  liquid  until,  after  70  to 
90  days,  the  liquid  becomes  perfectly  clear, 
while  a  viscous  network,  abont  0,5  cm.  high, 
is  formed  on  the  bottom  of  the  balloon. 
The  very  same  aspect  of  the  culture  is 
repeated  whenever  we  use  this  medium  for 
sowing  the  parasite. 
Medium  for  conservation. 
Poor  cultures,  even  when  showing  the 
aspect  of  the  cultures  in  Sabouraud  with  mal¬ 
tose. 
Sabouraud  with  raw  sugar. 
Abundant  cultures.  From  the  beginning 
they  look  like  the  cultures  of  Sabouraud  with 
maltose,  however  without  their  characteristi- 
cal  development. 
Fermentation 
Pellicle 
Gases 
Saccharose 
Yes 
Yes 
No 
Galactose 
« 
No 
« 
Nutrose 
« 
Yes 
« 
Milk 
« 
« 
« 
Mannite 
« 
No 
< 
Levulose 
« 
« 
« 
Bassiekow  I 
« 
« 
« 
Bassiekow  II 
« 
« 
various 
Bread 
fi 
(dense,  flocculent) 
No 
Raffinose 
« 
Yes 
« 
Dextrine 
< 
« 
« 
