10 
Glycose  broth. 
Culture  abundant,  appearing  soon.  Various 
forms  of  the  parasite.  Pellicle  thick,  brownish- 
white.  Deposit  abundant.  Good  medium  for 
the  study  of  the  fungus  in  hanging-drop  cul¬ 
ture. 
Media  containing  blood. 
(Sabouraud,  broth,  agar-agar,  etc.) 
The  culture  of  the  parasite  is  not  grea¬ 
tly  modified  by  adding  blood,  and,  in  our 
opinion,  these  media  offer  no  advantage. 
Drigalski— Conradi. 
The  parasite  develops  very  well  in  this 
medium.  Same  aspect  as  in  Sabouraud  with 
maltose,  except,  perhaps,  for  the  colour,  which 
here  is  violet. 
The  thick,  high  and  crinkled  culture, 
contrasting  with  the  medium,  is  thoroughly 
stained,  not  only  by  reflexion  of  the  medium. 
(This  phenomenon  was  seen  before  in 
other  brasilian  mycoses).  It  is  a  good  medium 
for  the  diagnosis  of  the  parasite  in  suspect 
sputa  and  we  may  say  the  same  of  the  me¬ 
dium  of  ENDO,  though  with  some  restricti¬ 
on,  because  the  finer  constitution  is  liable  to 
alteration.  The  cultures  here  become  reddish  ; 
they  are  thick,  velvety,  abundant  and  rapidly 
formed. 
Poor  medium. 
This  medium  is  suited  to  the  study  of 
the  sporulation  of  the  fungus;  it  was  propo 
sed  by  Mile.  GORODKOWA  (1908)  to  subs¬ 
titute  that  of  ENGEL-HANSEN. 
It  is  more  simple  than  the  latter  and 
does  not  need  cryslalizers,  HANSEN’s  vi¬ 
als  or  other  implements,  not  used  in  the 
common  cultures  of  fungi.  Germination  begins 
soon.  The  medium  being  rapidly  exhausted, 
the  development  of  the  parasite  becomes  re¬ 
markably  slow.  The  medium  has  the  ordinary 
aspect  of  the  Sabouraud  with  maltose  and 
may  be  used  for  the  study  of  the  biology  of 
the  fungus. 
LOEFFLER’s  Medium. 
Development  is  easily  obtained.  Cultures 
crinkled  and  velvety.  The  medium  is  impor¬ 
tant  for  comparative  study  of  the  morphology. 
(The  forms  of  the  fungus  are  similar  to 
those  of  the  sputum). 
Alkaline  Sabouraud. 
The  O.  brasiliense  develops  abundantly 
and  quickly  in  intensively  alkaline  Sabouraud 
with  maltose.  The  cultures  are  thick,  velvety, 
and  similar  to  those  on  the  classical  Sabou¬ 
raud  with  maltose.  The  medium  is  useful 
for  differential  diagnosis. 
Exactly  the  contrary  happens  in  acidified 
Sabouraud.  Here  the  parasite  develops  with 
difficulty  or  not  at  all.  The  media  made  with 
various  vegetables  are  favourable  for  the  deve¬ 
lopment  of  the  fungus. 
We  tried  the  O.  brasiliense  in  cul¬ 
tures  by  the  classical  methode  for  the  Spo- 
rol.icha,  i.  e.  cultures  on  slides  (BEURMANN 
&  GOUGEROT).  It  did  not  develop  well 
in  them.  In  hanging  drop  preparations  the 
growth  stops,  when  the  medium  becomes  dry. 
Microscopical  examination  of  the  arti¬ 
ficial  cultures. 
The  microscopical  examination  of  the 
O.  brasiliense  is  very  instructive.  The  first  dif¬ 
ficulty  is  to  obtain  a  regular  process  of  fixa¬ 
tion  and  staining,  for  few  processes  give  a 
good  and  clear  view  of  the  parasite. 
The  fixation,  which  gave  us  the  best  re¬ 
sult  for  simple  smears,  was  made  with 
SCHAUDINN’s  sublimate-alcohol,  either  hot 
or  cold  (wet  smears).  The  fixation  by  abso¬ 
lute  alcohol  can  be  applied  to  wet  and  dry 
smears. 
The  cells  and  the  mycelia  of  the  parasi¬ 
te,  fixed  by  these  two  processes,  are  as  well 
conserved,  as  one  might  wish  for.  The  mor¬ 
phology  is  equal  to  that  found  in  han¬ 
ging  drop.  We  also  experimented,  though 
with  inferior  results,  osmic  acid,  methyl  alco¬ 
hol,  heat  etc.  The  stains  of  the  smears  may 
vary  a  good  deal.  The  best  stain  for  the  cy- 
