14 
c)  subcutaneous 
d)  intraperitoneal 
e)  tracheal 
f)  buccal 
g)  nasal 
h)  bucco-nasal 
i)  pharyngeal 
The  age  of  the  culture  may  affect  the 
pathogenic  power  of  the  parasite.  Cultures 
on  Sabouraud  with  maltose,  dating  from 
more  than  a  year,  proved  virulent  for  la¬ 
boratory  animals.  We  noted,  however,  that 
the  virulence  of  the  parasite  grach  a  ly  di¬ 
minishes  with  age.  The  pathogenic  power 
of  a  fresh  culture  (of  two  months)  is  not  the 
same,  but  rather  stronger  than  that  of  a  two 
years  culture.  Simple  painting  of  the  buccal 
mucosa  (without  scarification )  with  culture- 
emulsion  in  physiological  salt  solution  kills 
the  animal  by  the  experimental  disease.  This 
power  of  penetration  of  the  Oidium  brasili- 
cnse  is  highly  interesting  and  explains  clear¬ 
ly,  how  the  human  disease  is  caused.  The 
five  monkeys  (4  Hapale  penicillata  and  1 
Alouata  fusca),  inoculated  in  this  way,  all 
died  with  typical  lesions,  though  after  va- 
rying  periods.  The  intermuscular  way  also  is 
exceedingly  important,  as  it  led  us  to  the  well 
founded  conviction  that  the  Oidium  brasi¬ 
liense  has  a  predilection  for  the  lungs. 
The  tendency  of  the  parasite  to  pulmo¬ 
nary  localisation  is  a  fact.  Some  animals,  in¬ 
fected  by  intramuscular  way,  died  with  lesi¬ 
ons  limited  to  the  lungs,  and  only  from 
these  could  we  recover  the  parasite.  The 
smears,  cultures,  etc.  of  the  points  of  ino¬ 
culations  never  gave  the  Oidium  brasiliense. 
This  fact  is  the  more  interesting,  as  we  know 
that  the  lung  localisation  of  the  fungus  is 
the  chief  manifestation  of  the  human  disea¬ 
se.  After  a  long  time  of  study  we  find  it  di- 
ficult  to  state,  which  of  the  laboratory  ani¬ 
mals  is  the  most  sensitive  for  the  Oidium 
brasiliense. 
We  prefer  the  sagui n  ( Callithrix  iacchus, 
to  any  other  animal.  Injected  with  0,5  ccm. 
of  emulsion  of  the  fungus  by  intraperitoneal 
way  (fresh  culture  of  two  months  of  age)  the 
saguins  may  die  within  22  hours  from  sep¬ 
ticemia.  With  cultures  of  2  years,  death  insues 
later,  sometimes  only  after  several  months. 
In  either  case  the  experimental  disease  is 
chiefly  characterized  by  its  attacks  on  the 
serous  membranes,  the  lymphatic  glands  and 
the  lungs;  the  inflammation  ot  the  former 
is  a  rule,  almost  without  exception,  in  the 
experimental  disease. 
Even  in  the  forms  of  rapid  septicemia 
(22  hours)  the  serosae  are  not  spared.  These 
lesions  of  the  serosae  in  the  beginning  of 
the  experimental  infection  corroborates  the 
suspicions  gathered  from  observation  on  pa¬ 
tients,  that  the  first  phenomenon  of  the  dise¬ 
ase  is  a  serositis. 
Another  important  fact,  shown  by  the 
experimental  disease  of  the  saguin,  was  the 
explication  of  some  lesions  of  lymph  glands. 
In  post-mortem  examinations,  performed  in 
fatal  infections  with  Oidium  brasiliense,  the 
tumefaction  of  the  mesenteric  glands  someti¬ 
mes  called  our  attention.  We  succeeded  in 
reproducing  this  glandular  swelling  in  the 
saguin.  We  give  a  photograph  of  the  me" 
sentery  of  one  of  these  animals,  infected  by 
the  peritoneum  with  a  three  years  culture  of 
Oidium  brasiliense.  It  shows,  how  the  swel¬ 
ling  of  the  mesenteric  glands  in  man  may 
find  its  explication  by  the  point  of  penetra¬ 
tion  of  the  germ  (intestine,  peritoneum).  From 
these  glands  and  from  the  liquid  of  the  se¬ 
rosae  new  cultures  of  the  fungus  were  direc¬ 
tly  isolated. 
In  the  septicemic  forms  we  obtained  in 
the  first  attempt  pure  cultures  of  the  parasite 
from  all  the  organs  of  the  saguin.  In  the 
chronic  form  we  find,  as  ride,  remarkable 
congestion  of  the  lungs  and  varying  degen  - 
ration  of  the  organs.  In  septicemia  there 
are  microscopical  abscesses,  chiefly  in  the 
lungs,  kidneys  and  spleen,  besides  various 
degenerations  of  other  viscera. 
The  infected  Callithrix  does  not  show 
anything  remarkable  in  its  clinical  aspect, 
wtiile  it  is  not  so  in  the  case  of  Hapale  and 
A  louata. 
The  clinical  aspect  of  the  experimental 
disease  in  the  latter  monkeys  is  of  great 
