15 
importance.  Shortly  after  the  painting  of  the 
bucco-nasal  mucosa  (without  scarification)  the 
experimental  disease  begins  with  progressive 
emaciation  of  the  animal.  Cough  follows  and 
the  monkey  takes  little  food.  The  facies  is 
emaciated  and  tbe  expression  is  torpid.  The 
animal  becomes  indolent.  Little  by  little,  the 
wheazing  first  noted  gives  way  to  subdued 
groaning.  The  animal  lies  in  a  heap  at  the 
farer  end  of  the  cage.  The  anorexia  is 
next  to  absolute.  The  coughing  fits  be¬ 
come  frequent  and  endless.  Cachexia  sets  in. 
After  one  or  two  months  the  animal  suc¬ 
cumbs  in  a  state  of  extreme  emaciation. 
So  died  the  5  monkeys  of  the  two  lattei 
species,  injected  with  the  Oidium  brasiliense. 
The  lungs  were  teaming  withe  the  fungus. 
The  white  rat  also  is  well  suited  for 
these  researches.  The  time  of  evolution  of 
the  disease  varies.  It  presents  nearly  always 
a  polyorrhomenitis.  In  the  serous  fluid  there 
are  characteristical  forms,  similar  to  those  of 
cultures  grown  on  media  either  poor  or 
exhausted  in  course  of  time  (abnormal  forms). 
They  are  equal  to  those  seen  in  GOROD- 
KOWA’s  medium  and  are  anomalous  ele¬ 
ments.  A  large  spherical  cell  with  double 
outline  and  homogeneous  protoplasm  gives 
birth  to  very  small  gemmules.  There  are  in¬ 
dications  of  mycelia,  only  0,5  micra  wide 
and  1  or  2  micra  long,  also  yeasts  like  the 
elliptic,  pasteurian  and  spherical  forms,  fre¬ 
quently  very  numerous.  The  protoplasm 
of  the  smallest  yeasts  stains  badly.  Such 
forms  of  the  fungus  are  seen  in  any  exsudate, 
while  its  duration  is  indicated  by  their  abun¬ 
dance.  It  is  of  importance  to  know  that  in 
liquids  of  long  standing  and  in  residues  of 
old  effusions,  there  are,  besides  the  descri¬ 
bed  forms,  mycelia  of  varying  aspect.  In 
the  effusions  of  human  serosae  we  also  ob¬ 
serve  a  strange  aspect  of  the  parasite.  This 
similarity  of  the  forms  from  the  serous  mem¬ 
branes  and  those  from  poor  media  deserves 
consideration,  as  indicating  that  the  serosae  are 
not  a  good  medium  for  the  growth  of  Oidium 
brasiliense.  KLECKI  observed  already  that  the 
virulence  of  some  bacilli,  obtained  from  the 
peritoneal  cavity,  is  weakened.  DIEULAFOY 
bases  upon  such  facts,  although  with  some 
reserve,  the  explication  of  what  he  calls  “de¬ 
ceiving  calm”  in  the  chapter  referring  to  ap¬ 
pendicitis.  The  Oidium  brasiiiense,  virulent 
in  the  circulation  and  in  the  organs,  is  to 
some  extent  weakened  in  the  serous  mem¬ 
branes  of  certain  animals.  The  pleural  stage 
of  the  human  infection,  primitive  in  our  opi¬ 
nion,  is  a  calm,  if  not  unperceived  stage. 
The  white  rat  is  suited  for  inoculation  of  the 
sputum.  The  lungs  are  always  affected. 
We  obtain  a  second  passage  by  injecting 
recovered  cultures  and  triturations  of  the  lung. 
There  are  cases  of  intense  swelling  of  the 
tracheobronchial  glands.  The  spleen  and 
the  liver  are,  as  a  rule,  swollen. 
The  rabbit  is  very  susceptible  to  infec¬ 
tion  with  Oidium  brasiliense.  The  experimen¬ 
tal  disease  in  this  animal  shows  two  forms; 
the  acute  and  the  chronic.  We  succeeded  in 
accompanying  the  evolution  of  the  disease 
in  a  rabbit  for  1  year  and  80  days,  while  ir 
an  other  case,  death  ensued  within  5  days. 
The  intravenous  infection  produces  in 
this  animal  a  septicemia  with  microscopical 
abscesses  scattered  all  over  the  organism. 
The  polyserositis  is  not  so  common  here,  as 
in  the  rat  and  the  monkey.  The  spleen  is 
sometimes  enormous.  The  suprarenal  capsu¬ 
les  are  almost  always  increased  in  size.  The 
lungs  show  from  smallest  congested  zones  to 
inflammations  of  basis,  apex  and  whole  lungs 
or  cavernae  (Note  1).  In  the  long  lasting  case 
just  mentioned  the  lung  of  the  rabbit  was  redu¬ 
ced  to  strings  of  connective  tissue,  joining  the 
two  innei  sides  of  the  thoracic  walls.  The  tra¬ 
cheobronchial  glands  sometimes  reach  the  size 
of  a  mediastinal  tumour.  This  tumefaction  is 
the  rule  in  the  infection  by  the  trachea  andt  he 
bucco-nasal  mucous  membrane  and  ressem¬ 
bles  the  enormous  tracheo-bronchial  adenopa¬ 
thies  found  in  people  who  died  from  infec¬ 
tion  with  O.  brasiliense.  Here,  as  in  the  me¬ 
sentery,  the  point  of  penetration  of  the  para- 
1)  The  tendency  of  chronic  lesions  in  man  is  hépati¬ 
sation  and  cavern  formation. 
