26 
identical  to  those  of  the  initial  cultures  on 
LOEFFLER’s  medium.  Rarely  they  are  sphe¬ 
rical.  In  these  forms  the  double  outline  of 
the  membrane  appears  with  considerable 
clearness.  The  yeast  forms  are  sometimes 
very  abundant.  Our  photograph  gives  an 
idea  of  the  degree  they  may  attain.  Such 
sputa  genera'ly  give,  on  first  sowing,  pure 
cultures,  showing  typical  gemmulating  forms. 
There  is  no  septation.  These  forms  show 
some  details  of  structure,  when  the  sputum 
is  fixedy  by  alcohol  and  stained  by  GIEM- 
SA’s  method.  The  distribution  of  the  chro¬ 
matin,  however,  is  here  of  little  importance. 
We  never  observed  the  classical  aspect  of  the 
Endomyces  albicans  (in  the  smears  of  mate- 
nal  from  scrapings  of  the  mouth);  but  this 
means  nothing,  because,  as  is  well  known, 
in  post-tvphous  pulmonary  endomycosis 
(GARIN)  this  parasite  presents  itself  in  the 
sputum  only  in  the  yeast-form. 
It  is  a  pity  that  GARIN  does  not  give 
more  details  on  this  form  of  the  parasite. 
One  fact,  however,  immediately  calls  our 
attention.  This  author  says  that  he  did  not 
find  mycelian  forms  in  the  smears  of  sputum 
from  patients  suffering  from  pulmonary  en¬ 
domycosis.  There  are  also  forms  which  we 
only  found  cited  by  HANSEN  in  some 
yeasts.  They  are  worth  noticing. 
There  exists  a  real  and  stainable  halo,  a 
gloea,  round  the  yeasts.  They  are  pulmo¬ 
nary  forms,  detached  and  expelled  by  cou¬ 
ghing  with  the  sputum.  The  colour  of  the 
gloea  is  different  from  that  of  the  parasite. 
With  S  AH  Li's  stain  the  involucre  appears 
pale  purple,  whereas  the  yeast  is  stained  in¬ 
tensely  blue.  This  gloea,  observed  in  pulmo¬ 
nary  foci  and  in  certain  artificial  cultures  of 
the  parasite,  is  a  product  of  secretion  of  the 
fungus.  HANSEN,  who  was  the  first  to  stu¬ 
dy  it,  obtained  it  by  drying  the  yeast.  It  is  a 
mean  of  defense  for  the  fungus  (there  are 
other  cases  known  in  mycology')  and  heips  in 
resisting  to  the  reaction  of  the  organism  or 
to  an  unfavourable  medium. 
In  the  Oidiuni  brasiliense  (sputum)  the 
mycelian  forms,  which  we  consider  rare,  and 
the  yeast  forms  are  seen  together,  even  on 
the  same  slide.  These  aspects  of  the  Ende- 
my  ces  albicans  and  of  the  Oidiuin  brasiliense 
are  very  interesting.  They  indicate  a  special 
pulmonary  form  for  both.  The  preparation, 
the  photography  of  which  we  give,  also 
shows  that  the  yeasts  are  nearly  all  of  the 
same  size  (about  3  micro).  The  same  forms 
of  the  Endomyces  albicans,  found  in  stomati¬ 
tis  and  lung  affections,  do  not  present  this 
uniformity  nor  have  they  the  same  size.  They 
rarely  reach  8  micra,  being  mostly  5  to  7, 
rarely  only  3  micra. 
The  mycelian  forms,  found  in  the  spu¬ 
tum,  have  nothing  characteristical.  They  are 
fragments  of  mycelia,  similar  to  those  found 
in  the  cultures  of  the  parasite.  We  ought 
not  to  confound  these  mycelian  forms  with 
other  similar  forms,  also  found  in  the  spu¬ 
tum,  though  of  different  nature.  The  inter¬ 
pretation  of  these  forms  of  sputum  is  inter¬ 
esting.  The  true  pulmonary  forms  are  not 
abundant  and  are  extraordinarily  adherent  to 
the  pulmonary  parenchyma,  some  of  them, 
however,  being  detached  casually.  They  are 
identical  to  those  of  the  poor  media. 
What,  however,  prevails  in  the  sputum, 
is  the  yeast  form,  with  rare  mycelian, 
generally  bronchial,  forms.  The  bronchi 
are  a  less  poor  medium  for  the  fungus  than 
the  pulmonary  medium,  being  similar  to 
LOEFFLER’s  medium.  The  fungus,  leaving  the 
parenchyma,  finds  in  the  bronchial  a  more 
apropriate  region  for  its  evolution.  Besides  the 
pulmonary,  there  is  a  bronchial  disease.  The 
expectoration  shows  much  larger  quantity  of 
bronchial  forms. 
Once  for  all,  we  declare  here  that  our  only 
aim  is  to  expose,  correctly  and  faithfully, 
what  we  succeeded  in  observing,  without 
other  intention  but  to  state  the  truth.  Theo¬ 
ries,  facts  or  opinions  do  not  preoccupy  us 
in  these  matters,  where  one  says  already  a 
good  deal  by  saying  what  one  saw. 
In  the  tissues. 
The  research  of  the  parasite  in  the  tis¬ 
sues  offers  some  difficulty,  because  of  the 
