134 
tained  air  bubles.  The  caudal  ends  were 
rather  retracted  and  difficult  to  perceive. 
On  the  17th  (the  next  day)  there  was  a 
very  characteristic  papule  on  my  arm.  1  re¬ 
moved  the  central  crust  and  expressed  a 
drop  of  serum  ;  in  the  afternoon  it  occurred 
to  me  to  cover  the  droplet  on  the  arm  with 
a  cover  glass.  A  still  quite  slender  and  al¬ 
most  colourless  breathing  tube  which  showed 
distinct  movements  was  then  projected.  The 
same  process  enabled  me  to  see  the  equally 
slender  and  colourless  posterior  end  of  ano¬ 
ther  larva  in  the  dog. 
Next  day  (on  the  18th)  1  tried  to  ob¬ 
tain  the  emergence  of  the  larva  which  provo¬ 
ked  a  slight  itching,  in  my  arm,  by  covering 
it  with  gelatine  and  agar.  It  projected  the 
posterior  end  but  was  unable  to  come  out 
and  remained  motionless  for  some  time.  I 
then  removed  it  by  pressure  which  some¬ 
what  damaged  it.  Its  appearance  was  almost 
like  that  on  the  first  day  and  there  was  no 
sign  of  a  moult.  In  the  morning  only  one 
of  the  larvae  was  visible  in  the  dog;  it  look¬ 
ed  very  much  larger. 
On  the  20th  the  larvae  in  the  dog  show¬ 
ed  tracheal  openings  and  very  much  enlarged 
posterior  ends. 
I  was  able  to  obtain  two  eight  days  old 
larvae  ;  one  whole,  the  other  in  fragments. 
They  had  moulted  and  were  very  much 
longer;  the  posterior  part  especially  had 
grown  in  every  direction.  The  whole  larva, 
observed  for  some  time,  at  the  temperature 
of  the  room,  showed  no  signs  of  life.  It  was 
7  mm  long.  The  exsudation  of  the  sac, 
occupied  by  the  larva,  was  mixed  with  pus. 
On  the  22d,  another  larva  was  observed, 
but  it  was  impossible  to  extract  it,  even 
after  inicision  of  the  sac.  The  larva  was 
obtained  the  next  day,  by  plugging  the  hole. 
The  part  of  the  body  covered  with  spines 
was  much  shorter  than  the  last,  very  elon¬ 
gated  segments  The  posterior  ends  of  the 
large  tracheae  wereyellow.  The  maggot  had 
attained  one  centimeter  oflenght,  thus  sug¬ 
gesting  that  the  stouter  thorny  part  must 
have  reached  the  subcutaneous  tissue.  The 
skin  of  the  larva  was  entire  but  eviscerated. 
The  viscera  were  recovered  seperately. 
A  short  time  after  this,  o><r  artist  caught 
a  fly  on  a  fazenda  in  the  Serra  da  Bocaina, 
on  wh'ch  he  saw  the  caracteristic  spot  due 
to  a  cluster  of  Dermatobia  eggs.  It  was  an 
Anthomyia  which  often  seeks  out  man  and 
animals  so  as  to  lick  their  sweat;  the 
species  was  probably  A.  lindigu  SCHINER. 
The  cluster  it  carried  was  composed  of  se¬ 
venteen  eggs,  attached  to  the  abdomen  near 
the  middle  of  the  left  latero-inferior  region.  It 
remained  alive  for  a  day,  dying  afterwards. 
The  eggs  showed  no  signs  of  life;  their 
brownish  colour  deepened  and  they  shrivel¬ 
led.  On  opening  them,  I  found  dead  larvae, 
which  had  probably  been  unable  to  reach  a 
suitable  host  in  time.  In  fact  the  fly  was 
caught  far  from  a  pasture  but  near  running 
water.I  kept  the  fly  and  the  egg  cluster  sepa¬ 
rately. 
On  the  occurrence  of  Oestrus 
ovis  in  Rio  de  Janeiro  and  the  neigh¬ 
bouring  states. 
On  Sept,  the  30th,  1916,  I  received  a  fly 
caught  by  Mr.  A.  LUCE  in  the  Rua  S.  Franc is- 
!  co  Xavier,  in  a  suburb  of  Rio.  Recognizing  an 
Oestridae  and  knowing  that  there  were  some 
sheep  near  the  place,  I  immediately  compa¬ 
red  it  with  BRAUER’s  detailed  description 
of  Oestrus  {Rhino  est  rus)  ovis  and  found  that 
it  undoubtedly  belonged  to  this  species.  La¬ 
ter  on,  I  examined  two  sheep  which  had  died 
in  the  same  place,  showing  the  symptoms 
I  observed  in  severe  cases  of  Myiasis  oestrina 
but  found  no  larvae.  The  infection  was  pro  ■ 
bably  brought  from  a  fazenda  in  the  Serra  da 
Bocaina,  where  animals  descended  from  eu- 
ropean  stock  had  shown  the  same  sym- 
ptoms. 
I  searched  for  larvae  in  the  slaughter¬ 
houses.  At  that  time  none  were  found  in  Rio 
but  I  obtained  some  from  Petropolis  ;  they 
had  been  found  in  native  sheep  bought  in 
the  neighbourhood.  Later  on  I  also  received 
I  some  from  Dr.  ESPIRIDIÃO  QUEIROZ, 
i  physician  in  Tres  Corações  (Minas). 
Rhinoestrus  ovis  was  doubtlessly  impor- 
