210 THE MICROSCOPICAL NEWS. 
has its best effect with nuclei (either alcohol or chromic acid pre- 
parations) and unicellular organisms, bacteria of all kinds, colout- 
less blood-corpuscles, &c. Zvopeolin, Y, 0, 00, ooo No. 1, and 
ooo No. 2. ‘The first is good for human spinal cord hardened in 
chromic acid, and alcohol preparations of bone, the others serve 
for connective tissue, cartilege, nuclei, and bone. The colours are 
lemon-yellow, straw-yellow, orange, orange-red, and brown. Cvocein 
he has found to be a very useful medium. It colours bone, carti- 
lage, muscle, and connective tissue (whether fresh or alcohol or 
chromic acid preparations) a beautiful purple-red. Poce//in colours 
bone and connective tissue, muscle, glands, and epithelium cherry- 
red. Xylidinponceau, Ponceau R R, G, and G G are not suitable 
for chromic acid preparations. The first gives good colours with 
bone, connective tissue, and muscle. The second gives red and 
scarlet-red colours. The third colours bone dark orange; connec- 
tive tissue, muscle, and epithelium saffron-yellow ; nerve substances 
bright yellow. The fourth has only been found useful for bone, 
gelatinous connective tissue, and muscle, which it colours a bright 
orange. Bordeaux R and G. colour the three last mentioned sub- 
stances, nuclei, and glandular tissue, the former giving a red and 
the latter a more yellow tint. Fresh are less successful than alcohol 
preparations. Bzebrich scarlet colours the most different tissues 
deep red. It is not suitable for chromic acid preparations. Cell- 
nuclei stand out sharply. Gold-orange serves for fresh or alcohol 
or chromic acid preparations, Bone is deep orange-red, cartilage 
gold, connective tissue reddish. It is especially valuable for glan- 
dular tissue ; it gives a splendid appearance to liver injected with 
Berlin blue, the blue vessels showing on a gold ground ; sections 
of skin give fine images. 
The preparations after washing and clearing are best mounted in 
balsam. Oil of cloves is mostly used for clearing. Very delicate 
colours are, however, often injured by the yellow of the oil of 
cloves, and in such cases oil of lavender should be substituted, or 
a quite colourless oil of aniseed. 
Dr. Griesbach gives a word of caution against the too hasty 
abandonment of the older media in favour of the new anilin 
colours, pointing out in regard to their use in permanent prepara- 
tions that our experience of their durability is not yet long enough. 
Whatever the future may bring, however, is this respect, they can- 
not fail to be of the greatest use in histology.—See 7 R.ALS., 
June, 1883. 
Mo.ver’s TypEN- AND PROBE-PLATTEN.—The catalogue just 
issued by Mr. J. D. Moller contains a somewhat startling item—a 
“type plate ” of 1600 arranged diatoms, the price of which is 1600 
marks or 80/.!_ With 800 or 400 diatoms, 20/. and 3/ 15s. is asked. 
