THE WILT DISEASE OF PIGEON-PRA, 3 
and chiefly consisting of Fusarium spores. The subculture was 
salmon-pink in colour, as the Fusarium beds on the bark of the wilted 
plants are. It was determined microscopically to contain both 
unicellular conidia formed at the tip of short lateral branches and 
elongated curved several-celled spores, and to agree entirely with 
the Cephalosporium and Fusarium stages of the Nectria. It bore 
no perithecia either then or in subsequent subcultures. 
The plants were grown in 24 flower pots, seeds, soil, and pots 
bemg unsterilised. 
The inoculation was made by sprinkling the culture, broken 
up in distilled water, over the seeds and soil, after sowing but 
before covering with soil, 12 pots being thus treated, while 12 were 
left uninoculated to serve as controls. 
No. of plants. | Treatment. pee, Oe: Result. | Remarks. 
32 plants in 12 | Inoculated | Sown and inocu-|1 plant wilted on All the remaining 
pots. with Fusarium| lated. 25-1-05. _ plants were killed 
and Cephalos- 22-11-04. |3 plants wilted on by frost on the 
porium spores. | 25-1-05. | night of 30-1-05, 
2 plants wilted on 
| 29-1-05. 
| {1 plant wilted on 
30-1-05. 
| 
27 plantsin 12 | Not inoculated 
pots. (control). Sown 22-11-04. No deaths up to 
| 30-1 05. 
This experiment showed that pure cultures of a fungus present 
on the bark of wilted pigeon-pea plants and agreeing with the Cepha- 
losporium and Fusarium stages of the Nectria found in the same 
locality are capable of producing wilt. Examination of the roots 
of three of the wilted seedlings showed them to be infested with 
hyphee in the manner above described, and copious Cephalosporiwm 
spores were developed on the surface of the roots when they were 
placed in a moist chamber for a few days. 
On February 25th the twelve pots that had been inoculated were 
resown with pigeon-pea. On April 19th two of this second batch 
of seedlings wilted in one pot. The roots of these were examined 
and showed clear signs of fungus-attack. Many of the lateral roots 
