4 E. J. BUTLER. 
were marked by shrunken blackened patches, in some cases near 
the tap root, in others some distance away. Most of the fine root- 
lets arising from these had been killed, and the appearances suggest- 
ed that the disease had originated in the latter and extended back- 
wards to the larger roots. The tap root itself was unaffected. The 
parasite, therefore, remains in the soil and is capable of infecting 
successive crops of pigeon-pea, a fact which is besides only too 
evident from field experience of the disease. Observation of the 
remaining seedlings ceased soon after April 19th owing to my absence 
from head-quarters. 
Series II. 
The culture used was the 5th successive subculture (on pigeon- 
pea slab) from an ascospore of the Nectria already mentioned. It 
contained Cephalosporium and Fusarium spores, but neither it nor 
the four previous subcultures had given rise to perithecia. 
The plants were grown in two pots in the same way as in Series 
I and sowing was done with germinated seeds. 
The inoculation was made after the seedlings were up by scrap- 
ing away the soil down to a lateral root and applying the culture to 
this. 
No. of plants. Treatment. rie ea peel Result. Remarks. 
4 plants in 1| Inoculated | Sown 25-2-05. No deaths up to On April lth made 
pot. with a_ sub- |[noculated 8-3-05. July 05. the number of 
culture from plants up to 6 with 
| Nectria. germinated  seed- 
oo ea ee ee ee | ee ee eee lings. 
6 plants in 1 Not inoculated | Sown 25-2-05. 
pot. | (control), | 
Pure cultures originating from Nectria ascospores, therefore, 
failed to produce the disease. 
Series ITI. 
The culture used was the same as that in Series IT. 
The plants were grown in 8 pots in the same way as in the pre- 
vious experiment, 
