12 E. J. BUTLER. 
Wilt appeared in pot No. V only. The subsequent history 
of the contents of this pot will be given below under Series XIV 
(p. 36). The plants from the remaining eleven pots were trans- 
planted into my garden on August 14th, havimg become too large 
for their pots. On September 8th those from pots II and III were 
attacked at the collar by a swarm of small red ants, and several 
were killed. One of these was pulled out on October 9th, and 
bore numerous perithecia of Neocosmospora on the roots. The 
rest remained healthy up to July 1908, and will be referred to 
again under Series XVII. 
Series VI. 
The cultures used were sub-cultures on nutrient agar, rice and 
onion from the rice tube used in the successful inoculation in Series 
V (pot V). 
The plants were those growing in pots I, HI, IV and VI of 
Series V. They were transplanted into my garden soon after in- 
oculating. 
The inoculation was made in the case of pot I by scraping away 
the soil from the base of the stem until lateral roots were exposed, 
scraping off the bark from these and placing a piece of the culture 
watered with distilled water on the wound, and in the case of the 
other three by simply watering the soil with the culture broken up 
in distilled water. 
No. of pot. | Treatment. Date sae Result. 
ion. 
| idl. Se ee 2 
I | Inoculated at a wound with 27-7-07. No deaths up to July 
| culture on rice. 1908. 
III | Inoculated by watering with 12-8-07. On September 8th several 
| vice, onion and agar cultures. plants killed by ants, 
rest remained healthy 
up to July 1908. 
Vv ‘9 o sf No deaths up to July 
1908. 
VI ” ” ” ” or) ” ” 
Though the plants in pot V of Series V, inoculated from a rice 
culture of Neocosmospora, contracted wilt, subcultures from the 
