14 E. J. BUTLER. 
The plants were grown in a small plot 8 by 4 feet, in my garden, 
a similar plot being sown as control. The plots were separated by 
a trench about a foot in depth. The seeds were sterilised by steep- 
ing in ‘1 per cent. formaldehyde for two hours. 
The inoculations were made by sprinkling the seeds and soil 
after sowing, but before covering, with the cultures broken up in 
distilled water. 
| Date of sowing and | Result 
No. of plants. Treatment. inoculation. 
Contents sa sera 8 by Inoculated by sprink-]Sown and inoculated | | Seven plants died 
4 fee ling with glucose | 18-7- as: between the 10th 
agar cultures, and 25th August 
14908. No more of 
| the several hun- 
dred plants died 
| up to the end of 
the year. 
| { 
| Not inoculated | Sown 1-7-08. No deaths up to 
(control). Ce tober 26th, 
8. 
| 
| 
| 
I am unable to explain the seven deaths in the inoculated plot. 
Not a single death occurred subsequently to August 25th, and this 
is almost conclusive evidence that the deaths were not due to true 
wilt. I have never seen a case of the latter where sooner or later 
neighbouring plants have not taken the disease. In a neighbour- 
ing plot affected by true wilt (that described under Series XVI 
below), deaths were numerous and spread was continuously going 
on up to early in the following year. 
Series IX. 
The culture used was a subculture originating from the first 
subculture from the plating in Series VIII. 
Subcultures were taken once a month and all gave very numer- 
ous perithecia during the four months which elapsed between Series 
VIII and IX. 
The plants were grown in pots which were immersed in ‘001 
per cent. corrosive sublimate solution for about five minutes before 
filling with soil. The soil was sterilised by intermittent steaming 
