16 E. J. BUTLER. 
in distilled water and crushed to obtain ascospores. These were 
plated. A good growth occurred in the trace of agar left on the 
walls of one of the tubes used for plating and was seen under the 
microscope to arise from a group of three or four ascospores. From 
this an agar tube was inoculated and the subculture thus obtained 
showed perithecia on the fifth day. Seven days later three sub- 
cultures were made from the first and gave microscopically pure 
growths, which bore numerous perithecia when examined after 
twelve days. Two of these tubes were used for the inoculations, 
the cultures being twelve days old. 
In the case of the gram Neocosmospora a plating was made 
of mature ascospores from a root of a wilted gram plant obtained 
from Bannu, North-West Frontier Province, and preserved in the 
laboratory for a year. Several good isolated Cephalosporium colo- 
nies resulted and a subculture from one, grown on agar, proved 
microscopically pure and bore numerous perithecia after five days. 
Subcultures were maintained regularly, all of which bore perithecia 
in large quantity. That used in the inoculations was five months 
removed from the plating and was about a week old. : 
The plants were grown in pots, the sterilisation of pots, soil 
and seeds being the same as in Series [X, except in the case of the 
indigo fungus where the pots were not sterilised. 
The inoculations were made as in Series IX, except in the case 
of the indigo fungus where the seeds were sown in pots after pull- 
ing out indigo plants which had been previously watered with the 
cultures broken up in distilled water. In this and im subsequent 
tables the control experiment, when one was employed, is always 
given immediately below the experiment to which it relates. 
No. of 
plants Treatment. Date of sowing Result. Remarks. 
| and inoculation. 
is 
16 plants in| Inoculated with | Sown and inocu- | No deaths up to 
2 pots. | cotton Neocosmo-| lated on 1-11-08. 5-38-09. 
spora. 
