22 E. J. BUTLER. 
pigeon-pea in the interval. The soil was mixed with enough fresh 
soil to fill five pots. 
The seeds were steeped in formaldehyde before sowing as 
before. 
. Date of 
No. of plants. Treatment. sowing and Result. Remarks. 
inoculation. 
About 50 plants in 6 | Inoculated with cotton | Sown and | No deaths | Transplanted to 
pots. Neocosmospora. inoculated upto my garden on 
3-4-08. Merck, 1-7-08. 
Do. do. Not inoculated (control) Sown ,, x a x. * 
17 plants in 2 pots. Inoculated with gram  Sown os aA 
Neocosmospora. | 28-3-08. 
| Inoculated 
1-11-08. | 
About 30 plants in Sown in soil previously | Sown 28-3-08.| Nodeaths Transplanted to 
pots. inoculated with indigo | upto my garden on 
Neocosmospora. Novem- 18-6-08. 
ber, 08. 
About 50 plants in 5 | Sownin soil previously - ne 
pots. inoculated with pig- 
eon-pea Neocosmo- | 
spora. 
Though the variety of cotton used in the experiments was one 
which is highly susceptible to wilt, inoculation with cultures deriv- 
ed from ascospores of Neocosmospora vasinfecta, whether from 
cotton, gram, indigo, or pigeon-pea, failed to produce the disease 
in a single instance. 
Indigo Wilt. 
About the middle of 1907 a serious disease of indigo appeared 
in Behar, the centre of the indigo industry of India. A few years 
previously the species of indigo known as ‘‘Java’’ (Indigofera 
arrecta Hochst.) was introduced experimentally into cultivation. 
Its superiority to the species previously grown, ‘‘ Sumatra ” (Indigo- 
fera sumatrana), was so marked that a rapid extension of its culti- 
vation occurred. The coincidence of the introduction of a new 
species and the appearance of a disease not previously known in the 
crop, was such as to lead to a strong suspicion that the disease had 
been introduced with the seed. Enquiry from Java, whence the 
