24 E. J. BUTLER. 
The culture was obtamed by planting ripe ascospores obtained 
from the root of a plant ma badly diseased field. On the fourteenth 
day a colony, bearmg perithecia, was selected and an agar tube 
inoculated with a trace of the aerial mycelium. This gave a pure 
growth which produced perithecia after five days. Subcultures 
from this were made and used for the moculations when three 
days old. 
The plants were grown in pots, pots and soil being sterilised 
as in Series XI (a). Half the number of pots were sown with see ! 
derived from plant imported from Java and grown for two genera - 
tions in India; the seed used having been carefully gathered from 
plants of the same field as that from which the fungus was obtained, 
and showing the earliest signs of disease. It was therefore of a 
susceptible stram. The other half were sown with Indigofera 
arrecta seed originating from Natal, and which had also shown 
itself to be susceptible. All the seed was treated with strong 
sulphuric acid for 20 mimutes. 
The moculations were made as before. 
(b) Indigo inoculated with Neocosmospora vasinfecta from cotton. 
The culture used was one of the regular series mentioned above, 
under Series X. It bore numerous perithecia. 
The plants were those in one of the control pots of (a) above, 
being Java seed two generations in India, and were nearly three 
months old. 
The moculations were made by scraping away the soil down to 
the roots and applying the culture broken up in distilled water. 
(c) Indigo inoculated with Neocosmospora vasinfecta from gram. 
The culture used was one of the regular series mentioned above, 
under Series X, and bore numerous perithecia. 
The plants were those in two of the control pots of (a) above, 
being Java seed two generations in India, and were nearly seven 
months old. 
