THE WILT DISEASE OF PIGEON-PEA. 27 
al 
but all the plants, inoculated ag well as control, died from heat, 
gram being a cold weather crop in India. They were resown at the 
beginning of the cold weather. At the same time a second series 
of inoculations was carried out, the sterilisation being very care- 
fully attended to. The seed used in both cases was local seed, as 
wilt occurs sometimes in the local crop. 
The inoculations were made in the usual way by sprinkling the 
seed and soil after sowing, but before covering, with the culture 
broken up in distilled water. 
(0) Gram inoculated with Neocosmospora vasinfecta 
from cotton. 
The culture was one of the regular series as before. 
The plants were grown in pots and sterilisation carried out as 
before. As in (a) the first sowings were killed by heat and 
resowings made at the beginning of the cold weather. 
The inoculations were as usual. 
(c) Gram woculated with Neocosmospora vasinfecta 
from indigo. 
The cultures used were part of a regular series maintained from 
the second culture mentioned under Series XII, 7.e., that derived 
from perithecia on the roots of Indigofera arrecta. 
The plants were grown and sterilisation effected as in (a) and 
(b) above. 
The inoculations were as usual. 
(d) Gram inoculated with Neocosmospora vasinfecta from 
pigeon-ped. 
The culture was the same as that used in Series [X above. 
The plants were grown, sterilisation effected and inoculations 
made as in (a), (b) and (c) above. 
