THE WILT DISEASE OF PIGEON-PEA. 33 
their mode of formation is shown in fig. 5, plate II, which may 
be compared with fig. 3, plate III of Smith’s paper. 
Chlamydospores are not uncommon in the vessels of old 
diseased roots, and are also formed on the surface and in old cul- 
tures. They are figured on plate IT, fig. 4, which may be compared 
with fig. 12, plate III of Smith. 
The cultural characters of Neocosmospora vasinfecta are very 
fully described by Smith. The greater part of his observations 
relating to pigment production were, however, carried out with 
cultures of the watermelon fungus originating from conidial forms 
on the host, not from ascospores ; there is, therefore, no certainty 
that they belonged to Neocosmospora. They never developed peri- 
thecia though continued for five years ona variety of media. Onthe 
other hand, his observations regarding the influence of different 
media on perithecia formation were made with ascosporal strains 
of the Cow-pea Neocosmospora. The following table gives a 
comparison between some of the results noted by Smith and 
those observed in ascosporal cultures of the Indian fungus from 
pigeon-pea, indigo and cotton. 
Cultural characters of American and Indian Neocosmospora from 
different hosts. 
Cow-pea (Smith). Pigeon-pea. Indigo. Cotton. 
Steamed potato. 6th) Steamed potato. In |Steamed potato. | 7| Steamed potato. 7 days, 
day, colour of sub-| about aweek surface| days, growth white| flocculent, growth 
stratum dark lead 
grey. Perithecia 
roseate by 6th to 9th 
day, then bright 
coral red. 
Pearl tapioca. 8th 
day, not 1/1000th as 
much growth as on 
potato. Perithecia 
few, appear slowly, 
at first pale red, 
then black. Re- 
main sterile. 
growth pink, no 
violet or purple. Be- 
low a_ pale green 
stromatic line. 
Brown stroma in 
contact with glass. 
Perithecia light red. 
14 days, perithecia 
darker, some brown. 
Tapioca. 
growth. 
14 days, no 
to dark greenish 
grey. Perithecia 
numerous, scattered, 
pale red to deep red. 
16 days, mycelium 
chiefly dark grey. 
Perithecia mostly 
rose. 
Tapioca. 7 days, no 
growth. 16 days, 
growth hardly visi- 
ble. No perithecia. 
white, stromata scanty 
pale green to _ pale 
purple. 16 days, peri- 
thecia many, bright 
red. 
Tapioca. 7 days, no 
growth, 16 days 
growth scanty. No 
perithecia. 
