THE WILT DISEASE OF PIGEON-PEA. 31 
and the fungus transferred in pure culture from a single-spore colony 
to an agar tube three days later. The resulting growth differed 
considerably in appearance from any stage of Neocosmospora, 
though on examination it was found to consist of Cephalosporium 
microconidia. Subcultures from this tube on steamed carrot and 
potato were used for the inoculations below. 
The plants were grown in pots sterilised by immersion in ‘001 
per cent. corrosive sublimate for about 5 minutes before filling 
with soil. The soil was sterilised by intermittent steaming on three 
days, the internal mass being maintamed at about 87° C. for two 
or three hours each day. The seeds were sterilised as usual by 
steeping in ‘1 per cent. formaldehyde for 2 hours. About 25 plants 
grew in each pot. 
The inoculations were made by sprinkling the seeds and_ soil 
with the cultures broken up in distilled water, after sowing but 
before covering. 
No. of pot. Treatment. Date of sowing Result. Remarks. 
and inoculation. 
1 Inoculated with | Sown and inocu- : Ist death on 
Cephalosporium | lated 13-6-08. Wilt appeared. | 16-7-08, typical 
from pigeon-pea. wilt. 
2 Ditto Ditto Ditto Ist death on 
1-8-08, typical 
wilt. 
3 i Ditto Ditto Ist death on 
ae 12-8-08, typical 
wilt. 
i Ditt Nitto lst death on 
‘ Ditto Bi ee typical 
wilt. 
Not inoculated Sown 13-6-08. ; No death up to 
(control). September 4th, 
08. 
ut 
6 Ditto Ditto Ditto 
