THE WILT DISEASE OF PIGEON-PEA. 
39 
or after some days in the moist chamber, nearly all the gram plants 
developed perithecia of Neocosmospora. From this it is apparent 
that Neocosmospora, though a saprophyte, penetrates freely into 
roots killed from other causes and fills the vessels with a mycelium 
closely resembling that of the true wilt fungus. 
SERIES XV. 
The culture used was obtained from the third batch of plants 
grown in the soil from pot V of Series V. A very recently attacked 
root was selected, washed carefully in several changes of distilled 
- water and rubbed well between the sterilised fingers to remove ad- 
hering soil and dead bark. It was then placed in distilled water 
on a sterile slide. 
In 24 hours a fine growth was visible on the sur- 
face and a trace of this aerial mycelium was transferred to an agar 
tube. On the 6th day there was a distinct growth along the streak 
and a subculture was made from one point on this to another agar 
tube. 
oculations after 13 days. - 
This proved microscopically pure and was used for the in- 
The plants were grown in pots, twenty-five seeds being sown 
im each. 
The seeds, soil and pots were sterilised and the inoculations 
made as in Series XIV. 
No. of pot. 
bo 
Treatment. 
Inoculated with 
Cephalospor ium 
from pigeon-pea. 
Ditto. 
Not inoculated 
(contro}). 
Ditto. 
Date of sowing and 
inoculation. 
Result. 
Remarks. 
Sown and inoculated 
17-4-08. 
Ditto. 
| Sown on 17-4-08. 
Ditto. 
Wilt appeared. 
Ditto. 
No death up to 
September, }j’08. 
Ditto 
Ist death on 
6-6-08, all dead 
by 12-9-08. 
Ist death on 
12-5-08, all dead 
by 8-9-08. 
