48 
ID OE ow 
— 
SS 
Ais 
12. 
E. J. BUTLER. 
Maltose 3% added to the standard solution. 
Glycerine 5% a 3 > 
Gum acacia 5% dy a 9 
Peptone 1% 93 9 > 
Asparagin 1% > 05 » 
Citric acid 1°5% ip >» » 
Sodium carbonate 1% _,, 5 99 
Absolute alcohol °5% _,, * Ms 
B. Solid media. 
Potato boiled in distilled water. 
Acid potato, prepared by soaking for 20 minutes in 1% 
sulphuric acid, then rinsing quickly in water and boiling in distilled 
water to sterilise. 
13. 
Alkaline potato, as 12, but 1% sodium carbonate used in 
place of sulphuric acid. 
14. 
15. 
16. 
17. 
18. 
Carrot boiled in distilled water. 
Acid carrot, as 12. 
Alkaline carrot, as 13. 
Rice boiled in a paste with distilled water. 
Acid rice, about 10 c.cs. rice moistened with distilled water 
and with 10 drops of °5°% hydrochloric acid added. 
i: 
20. 
Acid rice, as 18, but only 5 drops acid added. 
Alkaline rice, as 18, but 5 drops of saturated solution of 
sodium carbonate added in place of the acid. 
21. 
25. 
26. 
Alkaline rice, as 20, but only 2 drops alkali added. 
Plantain boiled in distilled water. 
Onion boiled in distilled water. 
Sugarcane pith boiled in distilled water. 
Tapioca boiled in distilled water. 
Nutrient agar; agar 15 grms., beef extract (Liebig’s) 4 
grms., peptone 10 grms., sodium chloride 5 grms., distilled water 
1,000 c.cs., neutralised by sodium carbonate. 
27. 
Glucose agar as last but with 20 grms., glucose added. 
The characters of the growth on these media are given below. 
