10 Tue MICROSCOPE. 
cerine, (Acetic acid 1 part, alcohol 70 and glycerine 30) the 
coloration takes place more quickly and in greater intensity. 
ACETIC ACID CARMINE. [BOHM’S CARMINE ACETATE.] 
Finely pulverized carmine is boiled in acetic acid (1 per 
cent.) until no more will dissolve. It is now allowed to cool 
and stand for a few days and then filtered. 
For showing the karyokinetic figures, the sections from a 
freezing microtome or the fresh object are placed in the acid un- 
til saturated, then washed in water and examined. Orif desired, 
they can be immediately mounted in glycerine or treated with al- 
cohol and clove oil in the usual manner and mounted in balsam. 
This fluid also largely takes the place of the dilute acetic acid of 
the laboratory for the light violet tint which it gives to tissues 
is a valuable help in studying its action. 
ACID BORAX-CARMINE, 
An aqueous solution of borax (4 per cent.) and carmine (4 
per cent.) is made by heating, and then acetic acid added, drop 
by drop, until the color has turned from a violet to a\red of 
about the intensity of Beal’s carmine. 
The above stains diffusely and cannot be employed for col- 
oring in mass. Sections are quickly colored by it, and then 
treated with acid alcohol. 
ALCOHOL CARMINE. 
Carniine 222220 2--. ~~. 2-222. 2-25 eee 4 grms. 
Alcohol) (60 percent.) -2-2 = 2 <t 22 ee 100 ¢. e. 
Hydrochloric acid... -- -.. ..- ee 30 drops. 
The carmine is dissolved and the solution neutralized by 
ammonia, which should be cautiously added that no excess is 
present. 
DOUBLE STAINS. 
CARMINE AND INDIGO-CARMINE, 
Carmine 42- <= -<==-~-+-- =i a ee 2 grms 
Borax 24. <2 2 es-2 2 352 - se as 8 grms 
W ater .2---2-]-- -- J. -- 2 eee 130 c. e 
(2) Indigo carmitie---...._.-____._-.._ 
IBOYax 22522 eset 2... ee eee 8 grms. 
W ater ic 2 eeaeee: - + 36a eee 130 ¢. © 
The solutions (1) and (2) are made separately and filtered, 
then mixed in equal volumes. Sections which are stained in 
about twenty minutes are then washed in a weak solution of 
