faik, MICROSCOPE. 
Vou. VI. ANN ARBOR, FEBRUARY, 1886. No. 2. 
ORIGINAL COMMUNICATIONS. 
HOW TO EXAMINE EPITHELIUM. 
Cc. H. STOWELL. 
COLUMNAR EPITHELIUM. 
a. samples of columnar epithelium remove a portion of the 
intestine of a recently killed rabbit or cat, and wash care- 
fully the mucous membrane by flowing over it some of the salt 
solution. Then with the point of a scalpel transfer a small 
portion of the membrane to a glass slide and to it add a drop 
of the staining fluid. Certain of the villi will be removed entire 
but a few of the columnar cells will be seen floating free in the 
field. An oval nucleus is present in each cell. These cells 
terminate usually in a fine point, a few are bifid, while others 
terminate in a rounded extremity. At the fine border of the 
cell is a thick margin in which free vertical striz are seen. If 
the animal be killed during digestion, globules of fat within the 
cells will be recognized by their strong refractive power. 
A few cells may be seen of pecul- 
iar chalice shape,called “ goblet cells.” 
Sometimes these are found quite 
abundant, but usually they are scat- 
ee tered here and there between the cyl- 
Pesice Se ieee eae indrical cells. The part of the cell 
cell.” x 400, nearest its free border has swoolen out 
owing to the conversion of its formed materialinto mucin. The 
contents of the cell, being hygroscopic mucin, swell up to an 
extent sufficient to burst through the free border, thus in normal 
conditions furnishing a certain amount of mucus. Frequently 
this structureless substance can be seen protruding from the rup- 
