Tur Microscope. 153 
drop of hzmatoxylin. This is allowed to remain until the sec- 
tion is stained a deep uniform color, when it is washed off. 
A drop or two of acetic acid is added and allowed to re- 
main on the section fora few moments. The strength of the 
acid used will depend upon 
the degree of coloring im- 
parted to the tissue by the 
staining. If the coloring 
was deep, the acid should 
be of full strength, if not 
so deep, then from a 10 to 
20 per cent. solution should 
be used. By this method 
the matrix is stained but 
slightly if at all, the cells 
more deeply, and the nuclei most intensely. The spaces be- 
tween the cells and matrix are now very distinct and the whole 
specimen shows everything to be desired. The sections are best 
preserved in glycerine. The hematoxylin should be yery 
strong for this process. 
Hyaline Cartilage. x 400. 
Chloride of gold is here also highly recommended. The 
section is placed in a 1 per cent. solution for 
15 or 20 minutes, then exposed to the light in 
distilled water for 24 to 56 hours, and finally 4 
mounted in glycerine. The cells are stained 
violet and they are not caused to retract from 
tint and the matrix is scarcely stained at all. “seas oo 
Osmic acid is useful in that it stains al]. Thytiod cartilage of 
the swine. The basis 
fatty matter black; it also stains the corpus- substance is divided 
into cell-districts by 
cles a deeper yellow than the matrix. means of chlorate of 
; : : A. Pl Da arety potash and nitric 
Picrocarmine is a satisfactory stain. acid. (Frey). 
Carmine will give good results if the sections be left in the 
staining for a sufficient time until they are stained a deep uni- 
form red color; the excess of carmine is washed away with 
water and a few drops of a 5 per cent. solution of glacial acetic 
acid are added. The sections are carefully watched under a low 
power, until the staining is seen to leave the matrix, when 
the acid is removed and glycerine added. It may be necessary 
