JEHANGIR FARDUNJI DASTUR. 187 



with earth from this diseased area. Care was taken to have the 

 topmost layer of the earth in the field uppermost in the pots 

 as well. Three seeds which were just germinating were removed to 

 three pots with a big lump of earth (Lot B). Ten seeds were sown 

 in each of the three pots of Lot A. On the 21st they germinated. 

 On the 22nd one seedling of Lot B was attacked with Phytophthora. 

 On the 23rd morning one of the seedlings of Lot A was suddenly 

 found to have given way at the collar, at which place there was 

 found an elliptical pale grey patch surrounded by a black brown 

 border which in turn was enclosed by a light brown and not 

 sharply defined ring. Sporangiophores were produced in a 

 large quantity, breaking out through the epidermis at any point. 

 On making sections internal sporangia were also found as far in 

 as the pith. The cells of the pith and cortex were traversed by 

 palmately branched or fasciculated hyphse, the like of which 

 have till now been observed only in some of the artificial media ; 

 wherever these hyphse were found, the cell walls between two con- 

 tiguous cells were partly or wholly dissolved (Plate II, Fig. 5). 

 These palmately branched hyphse have also been observed by 

 Mangin 1 in Plasmopora viticola, PI. ribi, PI. nivea and some 

 other Peronosporacece and according to him by MM. Milladet, 

 Prillieux and Cavara as well, but these hyphae, Mangin has found 

 only in tissues surrounded by fibro-vascular bundles. He says. 

 " cepeiidant les tissus compacts qui entourent les nervures et qui 

 cantonnent le mycelium dans des regions limitees, ne constituent 

 pas une barriere infra nchissable. En ces points, le mycelium s'aplatit 

 et forme des lames on des palmettes tres elegamment ramifiees, qui 

 s'insmuent dans les meats les plus etroits et parviennent meme, soit 

 niecaniquement, soit en digerant la substance intercellulaire, a dis- 

 socier un peu les cellules qui leur opposent de la resistance. La 

 moindre fissure, la moindre fente, suffisent pour que le mycelium 

 franchisse l'obstacle et penetre dans une region saine ; la. il eVolue 

 rapidement en pleine liberie an moyen des elements nutritifs qu J il 



I Mangin, L. Recherches sur les P6ronospor£es. Hull. Soc. d'Hist. Nat. d'Autun, VIII, 

 L895, p. Tl». 



