JEHANGIR FARDUNJI DASTUR. 197 



got entangled with some foreign body. It evidently tried to set itself 

 free. At 10-20 four vacuoles appeared, at 10-25 it succeeded in 

 disentangling itself and suddenly darted off. At 10-30 its motility 

 ceased, it lost its cilia and rounded itself off. At this stage only one 

 vacuole was visible. It measured 15 x 12m. Its endospore and 

 exospore were quite distinctly visible (Plate IV, Fig. 27). The for- 

 mer was hyaline and refractive, the latter dark. At 10-45 it began 

 to germinate. It produced three germ-tubes. Plate IV, Figs. 27 to 

 34 show its progress till 3 p.m., after which there was no further 

 development, possibly on account of lack of food. This motile mass 

 of protoplasm, having five visible cilia and going through the ordi- 

 nary life cycle of a normal zoospore and germinating by giving out 

 three germ-tubes, possibly consisted of three undifferentiated zoospore 

 constituents. Dr. Butler 1 has observed in Pythium palmivorum two 

 or more spores remaining united after leaving the sporangium and 

 germinating normally after coming to rest, but they have only a 

 single wall. 



The zoospores are of the ordinary Phytophthora type and measure 

 8-12 x 5-8« in their motile state, when they come to rest they measure 

 7-1 lu. They are bean-shaped with a pointed anterior ; arising from 

 the hilum are two unequal cilia, the anterior one being distinctly 

 shorter, as found in Ph. Syr'nigce 2 and Ph. omnivora, var. 

 Arecce' 3 (Plate IV, Fig. 6). After swimming about for twenty 

 minutes to two hours they come to rest. Their movements 

 are very brisk for the first fifteen minutes ; they move about 

 even after assuming the spherical shape, the movement then being 

 not very energetic. The rounded zoospore after rolling about for a 

 few seconds comes to a dead stop to all appearances, but quite 

 suddenly after a few seconds it dashes away, presumably in search 

 of another suitable resting place. This fitful and sudden move- 

 ment goes on for about five minutes more and then the spore 

 comes to a dead halt, hi fifteen minutes germination takes place 



1 Butler, E. J. Bud-rot of Palms in India. Mem. Dept. Agric. India, Bot. Ser. Ill, No. 4. 

 1910, p. 255. 



2 Klebahn, H. loc. cit., p. 51. 



s Coleman, L. 0. loc. cil. f p. 015. 



