JEHANGIR FARDUNJI DASTUR. 215 



water to keep it moist after sterilization. Aerial growth was as good 

 as on maize corn meal. A very poor crop of sporangia was produced 

 after a fortnight, but many ' ; resting " conidia were formed. Sporan- 

 gia! growth was as poor in the - 5 Fuller's scale bread paste. In 

 neutral and + 5 Fuller's scale media, sporangia- were produced in 

 a fairly good quantity. 



Ricinus leaf juice. — This medium was made by boiling about 15 

 grammes of green leaves in 100 c.c. of water. The growth was poor, 

 the mycelium had no irregularly budding hyphse. No sporangia 

 were produced. 



Ricinus leaf juice agar (100 + 7 + 500). — The growth was sub- 

 merged and localised. Vegetative hyphse branched in a coral-like 

 fashion. Sporangial formation was very poor. 



Ricinus seed juice agar (12 + 5 + 500). — This medium was pre- 

 pared as follows : 12 grammes of seeds, with their hard seed coats 

 removed, were slightly crushed and boiled in about 100 c.c. of water. 

 To the filtered decoction was added 5 grammes of agar, dissolved 

 in water and the whole mixture was made up to 500 c.c. The 

 fungus grew moderately well on this medium. Growth was con- 

 centric round the point of inoculation and it was entirely submerged. 

 The vegetative mycelium had only a few swollen or budding 

 hyphse. Sporangia were copiously produced. 



Peptone water {Dunham's solution). — There was a moderately 

 diffused mycelial growth. No sporangia were formed. 



Peptone Bouillon.— The growth was not so good as in Peptone 

 water ; the vegetative mycelium contained abnormally broad and 

 budding hyphse. 



Peptone broth. — The fungus grew very poorly on this medium. 

 Vegetative hyphse were irregularly branched and budding. Sporan- 

 gia were formed. The mycelium growing within this medium and 

 on several other media, resembled the sterile, aquatic mycelium of 

 Pythiacystis citwphthora, Smith and Smith, grown in dilute prune 

 juice agar (Plate Y, Fig. 10). 



Groundnut agar. — Twenty seeds of groundnut powdered in a mor- 

 tar were boiled in 1 50 c.c. water for two hours. The boiled mixture was 



