216 PHYT0PHTH0RA PARASITICA. 



allowed to stand for 24 hours. The supernatant liquid was filtered, 

 mixed with 7*5 grammes of agar dissolved in water and was made 

 up to 500 c.c. The mycelium radiated from the point of inoculation. 

 The growth was fairly good but submerged. The vegetative hyphse 

 were broad and budding and branched at right angles. They were 

 full of large oil granules. They presented a warty appearance on 

 account of many of the branches failing to make further progress 

 after they had originated. They were at some places so broad that 

 they looked as if they were galled. Sporangia were few and scattered. 

 Jowar (Sorghum vulgare) agar. — The medium was made up in 

 the same way as groundnut agar except that 1 ounce of well ground 

 jowar grains were used instead of groundnut seeds. The culture 

 on this medium was in every way the same as on groundnut agar, 

 except that there was a little aerial mycelial growth and that the 

 sporangiferous hyphae also branched and budded like the vegeta- 

 tive hyphae but not so much. 



Potato slab. — The aerial growth was quite luxuriant but was 

 never found to be fertile. The inoculation succeeded only on the cut 

 surface and not on the peel, just as in the living potatoes. 



French-bean powder. — The aerial growth was fairly good. Very 

 few sporangia were produced but there were many " resting " 

 conidia. 



Glucose meat-extract agar. — This medium consisted of : — 



Extract of Lemco 

 Sodium chloride .. 

 Peptone 

 Glucose 



Agar 



Water 



The growth was wholly within the medium and not localised. 

 The fungus gave the surface of the medium a roughened appearance 

 as if it had bacterial contamination. The hyphae were fertile, 

 irregularly branched and swollen as in groundnut agar and other 

 media . 



