JEHANGIR FARDUNJI DASTUR. 217 



Meat-extract gelatine. — The growth was very poor. No spo- 

 rangia were produced. 



Dolichos live beans. — The growth of the mycelium was over 

 one-third inch high. Sporangia and " resting " conidia wete formed 

 m abundance. 



Prune juice agar. — This medium was prepared by boiling prunes 

 in 100 c.c. water for 5 minutes and adding to the filtered decoction 

 I'o grammes of agar dissolved in water, making up the whole mixture 

 to 500 c.c. The growth was not very poor. The mycelium con- 

 tained irregularly swollen budding hyphee. Sporangia were poorly 

 formed. 



Prune juice agar acidified with citric acid. — On this medium the 

 fungus grew fairly well but the mycelium remained sterile even one 

 month after inoculation. The hyphse did not contain irregular 

 branches or abormal swellings. 



Sterilised slabs of carrot, sweet potato (Ipomoea batatas) and of 

 conns of Colocasia antiquorum did not give the slightest growth of 

 the fungus. 



Cultures were also made on sterilised flies and ants in sterilised 

 water but they gave a very poor growth of hyphse and sporangia. 1 



Inoculation Experiments with, and affinities of, Ph. parasitica. 



In order to test the affinity of Ph. parasitica with the various 

 species of this genus, a number of inoculation experiments were 

 undertaken on as many of their host plants or their allied species 

 as were available. Inoculations were invariably done by means of 

 suspensions of motile zoospores in distilled water taken from 

 pure cultures. The inoculated plants, seedlings and cuttings were 

 covered with bell jars and the inoculated fruits were placed in moist 

 chambers to keep them constantly in a moist atmosphere. The 

 " checks " were kept in exactly similar atmospheric conditions. 



Fruits of Cacao, two species of Oenothera and of Cere us were 

 obtained through the courtesy of Mr. C. Mahaluxmiwalla, Superin- 



1 Cf. Coleman, L. C. loc. tit., p. 71. 



