154 REICHKI, : KABIKS. 



To Dr. Anna Williams and May Miirra}- Lovvden, of the 

 New York Board of Health, )>n(st be given the credit for the 

 shortest method in the demonstration of Negri bodies. 



Small pieces of the brain tissue are taken, in which the 

 bodies are most frequently found, usually the hippocampus 

 major or the ce?-ebe!lu]n , and placed upon the end of a glass 

 slide and covered by another. The first slide is then drawn 

 toward the end of the second slide, spreading the tissue over 

 the slides in a thin layer, making two smear preparations. 

 The smears are then fixed upon the slides by placing the slides 

 in absolute alcohol for several minutes. For staining, Loef- 

 fler's alkalin methylene blue and basic fuchsin in the right 

 proportions are used. The slide is washed in water and dried 

 between filter paper, after staining, and examined microscop- 

 ically. 



The smear, properly spread and stained and examined, 

 shows the structure of the larger nerve cells stained a light 

 blue, the Negri bodies a pink or light red, with dark, granular 

 bodies in the centre. In this way Negri bodies can be dem- 

 onstrated in several minutes, and a diagnosis quickly estab- 

 lished. 



