1896 THE MICROSCOPE. Ill 



tog-ether with the fact that the Alternaria was found abund- 

 antly in every one of the diseased leaves examined, pro- 

 duced a strong impression that the disease was due to the 

 fung-us in question. There was no opportunity however, 

 to prove this supposition by the inoculation of sound 

 leaves with a pure culture of the Alternaria. When the 

 field was first examined, late in August, the plants had re- 

 ceived two applications of Bordeaux mixture, but it had 

 not been applied very evenly. The only thing- to be done 

 under the circumstances was to recommend another im- 

 mediate and thorough application of Bordeaux mixture, es- 

 pecially upon those portions of the vines at a distance from 

 the centre, which as yet showed no symptoms of the dis- 

 ease. There was but little hope however, of saving the 

 crop to any great extent, and as the result proved, the dis- 

 ease continued to spread even after thorough treatment of 

 the vines with Bordeaux mixture. 



There are a number of fungi which are known to affect 

 the leaves of melons, but the one in question is probably 

 identical with the species of Alternaria recently described 

 by Smith and by Peglion as affecting melon-leaves in this 

 country and in Italy. Whether in this case the fungus 

 was a true parasite, or whether it merely followed an in- 

 jury due to other causes, must remain an open question 

 for the present. — Exchange. 



Chloral Carmine.- — The following makes a beautiful and 

 permanent carmine useful in almost every kind of work, 

 and especially so in animal histology: 



Carmine, No. 40 50 cgm. 



Alcohol 20 ccm. 



Acid hydrochloric 2 ccm. 



Chloral hydrate 25 gm. 



Mix and dissolve. — Kai. Druggist. 



The Lepra Bacillus has been found in the blood, as 

 well as in the tissues, by Dr. Bouffe, of Paris. 



Epithelium in Urine. — Under the microscope this is 

 seen as irregularly shaped bodies. 



