"joumai,ST.mf 'J Cultivation of Microscopic Fungi. 17 



used. The edges of the cover, if wetted by the acetate sokition, 

 attract moisture readily ; so it is best, if the specimen be worth closing 

 up, to dry the edges by a sable pencil and twist of tissue-paper 

 before applying Bell's cement, at first thin, then thickened, as afore- 

 mentioned. 



On the 1st September, a few of the spores (conidia or sporanges) 

 were removed from the surface of a grape, covered with Oidiiim 

 Tuckerii, and sown as described in the cultivating solution, placed 

 in the porous cell in the moist chamber, and left in diffused light. 

 On the 4th there was an abundant raycehum from several of the 

 spores or conidia (Fig. 1 h), afterwards becoming of a light-brown 

 colour, divided by septa, enclosing generally two oil globules or 

 minute spores. Later, on the 15th, from the mycelium several stalks 

 had sprouted, and fruited into the air-space beyond the edge of the 

 liquid ; the fruit resembling Penicillium, but the spores in rows on 

 the pedicels were sHghtly oval. Some of the older stalks had a cha- 

 racter which I had not noticed before, and which I have endeavoured 

 to show in Fig. 1 d. They appeared rough outside, or covered with 

 most minute bodies. I am of opinion that this was not an accidental 

 character, but from absence was unable to trace this point further, 

 or whether such stalks might not break up into other bodies. 



Lying about amongst the threads were numerous minute bodies 

 (?)bacteria, also small oval bodies with a central spot or nucleus, 

 mostly collected into groups (Fig. 1 e). It is possible these may 

 have been drawn in from some slight shrinking of the fluid, and 

 have originated, as supposed, from the heads that fruited in the air- 

 space ; but whence they were derived I am uncertain. 



On August 30th, a small speck from a brand on an orange- 

 leaf (from the same greenhouse), which for trial had been set in 

 fresh gooseberry juice, was removed, as it had not altered, and set 

 in the cultivating solution, &c., in the light. The spores or conidia 

 commenced sproutiag very shortly after, and soon became filled with 

 oil globules or spores, as in Fig. 2 (/, h. On the 5th many of the 

 mycelium threads had formed heads outside the fluid, the spores 

 being very slightly oval (Fig. 2 i). 



In the fluid lying by the side of some of the original conidia 

 or spores which had sprouted were minute oval bodies with a central 

 nucleus in each (Fig. Ij). On the 1,5th the stems of the older 

 fruited threads appeared roughened on ths exterior, with minute 

 bodies, as in the former case. The young threads were growing 

 beautifully, and filled with closely-packed oil globules or spores, 

 no septa being distinguishable, while in the older mycehal threads, 

 which were of a brownish colour, septa existed; each division 

 inclosing about six small bodies (Fig. 2 k). 



Another slide was set from a black brand (found on a leaf of a 

 climbing plant, * * *^ in the same greenhouse) with the culti- 



VOL. III. c 



