Sutler and hafiz 185 



spores has been observed. The species does not agree with anv 

 previously described and has been named Cephalosporium Sac- 

 chari, the diagnosis being as follows : — ■ 



Cephalosporium Sacchm^i Butl. n. sp. EfFiisum, candidum ; 

 hyphis repentibus, parce septatis, 3-5 ^ diam. ; conidiophoris 

 continuis, simplicibus furcatis vel subverticillato ramosis, sursum 

 obtusis, medio vel versus basim incrassatis, 6-30 Mlong. 3-4 /x crass.; 

 conidiis ex apice raraulorum pluribus continuis exsilientibus et in 

 capitula coUectis sed facillime secedentibus, hyalinis, ovoideis vel 

 oblongo-ellipsoideis, continuis, 4-12 ^ 2-3 ,u. 



In culmis Sacchari offlcinarum in India or 



Cultures of the fungus can be readily obtained in the same 

 way as with Colletotrichum falcatum, by cutting out aseptically 

 a portion of the pith, in the early stage of the disease, and incubat- 

 ing in a sterile Petri dish or potato tube. The growth is frequent- 

 ly pure from the commencement, but if not, can be readily purified 

 by sub-culturing from the aerial mycelium. The following in- 

 oculations were carried out with pure cultures from nutrient agar, 

 in which spore-production was copious. 



On December 1 3th, 1908, 20 strong sound Red Mauritius 

 canes were inoculated in a stem wound with a suspension of spores 

 and mycelium in distilled water, from a culture two weeks old. 

 The inoculations were made in the lower half of the cane by 

 removing a cylinder of pith with a small sterile cork borer, insert- 

 ing a drop of the suspension, and replacing the cylinder after 

 cutting off a little of the end, so as to leave a small cavity. The 

 wound was then bound with sterile gutta-percha sheeting. 



The canes were examined after 3 months. In one case onh' 

 had the whole cane dried up, the infection extending above the 

 wound to the top of the cane, and below for 3 internodes. In the 

 others infection had progressed through from 4 to 6 internodes. 

 The inoculated internode had become hollow in every case, those 

 above and below being also hollow in some cases. The symptoms 

 were typical and the fungus was found throughout the discoloured 

 parts. 



