]\)C, NKW SUGARCANE DISBASES 



ill less than '2-4 liours by an outgrowth from one or both onds, tho 

 i,^prni-tube remaining unseptate until it has reached a consider- 

 able length (PI. V, Figs. 4 & 5). 



The other spore form (PI. V, Fig. "2), consists of hyaline 

 filamentous cells, usually without septa but with many oil drops, 

 straight or irregularly curved, variable in length and breadth, the 

 lono-p.ct bein<y often very narrow, sometimes broader at the base 

 and tapering to a narrow apex, sometimes quite uniform in 

 diameter. They measure 20 to 60 by 'G to '2 /n. Germination 

 was not observed. 



An intermediate form of spore (PI. V, Fig. 3) is sometimes 

 found, consisting of pale yellow, elongated, 1 -septate cells, borne 

 on sporophores of the type above described and measuring 18 to 

 :<0 by 2 to 3 "7 5 m- 



After the pycnidial stage had been obtained in pure cultures, 

 it was found in two cases on old canes attacked by the disease, 

 one beino- an inoculated cane at Pusa, the other a diseased cane 

 from Jorhat. The stromata develop in the internodes, under the 

 epidermis, or one or two laj^ers deeper in, and are smaller than 

 those obtained in culture. The outer fibrous layer is absent or 

 reduced, the mass of the stroma being parenchymatous. The 

 base is broad and extends for some distance all round, as a narrow 

 stromatic layer between the tissue cells. In the centre, the 

 epidermis is raised up and ruptured by the roughly conical, deep 

 portion of the fruit bod}^ which is hollowed out into one oi- 

 several cavities. Unilocular stromata are common, the cavity 

 being irregular or sometimes imperfectly divided by incomplete 

 walls. The mouth is usually single and develops late. Both 

 spore forms occur in the loculi. 



There is much greater uniformity in the spore characters in 

 any one culture of the fungus, than would appear from the above 

 description, which is based on the examination of a large series. 

 Thus, in some cases, the first spore form was almost entirely 

 unseptate and small and the second broad and distinct ; in others 

 bicellular spores of the first type were chiefly found, intermingled 

 with which were a few hyaline, attenuated spores of the second 



