BUTLER AND HAFIZ 199 



an ascigerous stage. After a week the onion cultures were the 

 best, followed closel}^ by carrot, potato, Carica, Colocasia, Psidium 

 and rice. On bread also the growth was good, while plantain 

 and sugarcane were much inferior, especially the last. The 

 mycelium was loose and had begun to lose its original white 

 colour in many of the tubes, the upper part of the aerial 

 mycelium (which exceeded 2 inches in height in some of the 

 cultures) turning grey, except in the plantain, rice and sugarcane 

 tubes, while the growth near the surface of the medium remained 

 white in all. A submerged growth had developed in the water 

 in the bottom of the (potato) tubes and this became light purple 

 or pinkish in the carrot, onion and Carica tubes. The medium 

 was discoloured dirty gre}^ in the onion tubes, eye-grey to purple 

 in those of rice and slaty-blue in those of bread. Five days 

 later a blackish crust had developed on the surface of the water 

 in the potato, carrot, onion, Carica and Psidium tubes, the 

 submerged mycelium was light cinnamon colour in the onion and 

 Psidium tubes, brick red in the plantain, and unchanged In the 

 others. The colour had diffused into the water, The aerial 

 growth was turning brown on the surface in the carrot, onion, 

 plantain, Colocasia, Carica, Psidium and bread tubes, with a 

 little pink in places in those of Colocasia. The medium was 

 blackened in the potato, carrot and Carica tubes, while the upper 

 part of the rice was dark coloured and a bluish line separated 

 this from the unaltered lower portion ; the same bluish colour 

 developed in the upper part of the bread paste. Seventeen days 

 later there was little change, the growth in the potato and onion 

 tubes was darker, that in the carrot, plantain, Carica and 

 Psidium tubes had become pinkish in places, the submerged 

 mycelium vvas chocolate-brown in the plantain, and chestnut in 

 one of the susfarcane tubes, and a few dark dots were noticed in 

 the denser parts of the mycelium of several tubes. These did not 

 develop further and were ultimately found to be merely 

 condensations of sterile mycelium. 



The following inoculations were carried out with pure 

 cultures, obtained as above described. 



