feUTLER AND HAFIZ 201 



On the same date as the last, a second series of inoculations 

 was carried out in the same plot, by removing the soil from 

 around the base of four clumps of cane and watering the exposed 

 roots with a suspension of the mycelium beaten up in water. 

 These also took well, and when seen early in March each clump 

 had (jne or more withered canes. No other canes were witherintf 

 in the rest of the row to which the clumps belonged nor, so far as 

 could be observed, in any part of the plot, except the row in 

 which the first series of inoculations had been made. One cane 

 from each clump was split and found to have typical symptoms of 

 the disease, but there was no opportunity for microscopic exami- 

 nation. The symptoms were, however, so definite that there 

 was no doubt that this series was quite as successful as the first. 



As the disease does not occur at Pusa, it was not considered 

 advisable to carry out any inoculations in the field. Some were, 

 however, made on plants grov/ing in tubs, at a distance from the 

 farm crop. The number of canes inoculated through stem wounds 

 was six, growing in four tubs, one tub being kept as a control. 

 The inoculations were made in November 1909, exactly in the same 

 manner as those of the first series at Samalkota, In the following 

 March none of the inoculated canes had withered and two were 

 removed for examination. The inoculated internode was found 

 diseased in both, the mycelium being confined to the one internode 

 in one case but extending to the next higher up in the other. The 

 other plants were kept in the tubs until the following year, by 

 which time they were still alive but had ceased growth, except for a 

 few feeble side shoots. In May 1911, they were cut and examin- 

 ed. The inoculated canes were much reddened near the wound, 

 the reddening extending for from 2 to 4 internodes each way. 

 Above the reddened part, the characteristic translucent appearance 

 and internodal hollowing of the pith was found. The red parts 

 were full of the hyphse of the parasite and, in one case, pycnidial 

 stromata had developed in the internode next below that inocu- 

 lated. The roots were not affected and no spread to other shoots 

 in the same stool had occurred. The parasitism was, therefore, 

 much less marked than at Samalkota, though the spread of the 



