80 The Mickoscope. 



substance of the membrane. Cut out a piece, including corpuscles, 

 make it tense on a stretcher, fix with pins at the four corners and 

 drop into Miiller's fluid. (6.) The oesophagus, stomach, intestines, 

 ileo-coecal valve and colon. Slit them open, cleanse gently and place 

 one inch pieces of each in alcohol. If desired, a short piece of intes- 

 tine can be w^ashed out with water and preserved without opening. 

 (7.) The pancreas, and (8.) the spleen. Preserve cubes in alcohol. 

 (9.) The testicles and penis. Remove the testicles from scrotal sac, 

 dissect out penis and put in alcohol. (10.) The tongue and larynx. 

 Remove the head by cutting down on the vertebral column from 

 behind. Disarticulate the jaw and the tongue and larynx can be 

 removed en masse. Divide the tongue in halves by a longitudinal 

 median incision and preserve in alcohol. The larynx should be 

 put in Miiller's fluid. (11.) Submaxillary gland. Remove and pre- 

 serve in alcohol. (12.) The brain. Remove the skin, then, with a 

 pair of nippers or forceps, snip away the bone, beginning at the ridge 

 of the nose. Care should be taken not to injure the brain or eyes 

 during the operation. With patience the brain will be finally uncov- 

 ered. With scalpel and scissors separate the attachments and nerve 

 connections ; lift out carefully, wash and divide by longitudinal in- 

 cision. Place in Miiller's fluid. (13.) The eye. A little more 

 careful snipping will expose one. Preserve, without opening, in 

 Miiller's fluid. (14) Nerve. At the base of the spine large nerve- 

 roots will be found. Cut out one or two of the large ones, place on 

 stretcher and immerse in Miiller's fluid. (15.) Muscle. Take a 

 piece from the thigh. An outer piece attached to the fascia can be 

 made tense on a stretcher and dropped into Miiller's fluid. Another 

 portion, unstretched, can be placed along with it. (16.) Bone. 

 Remove the thigh bone, clean, cut in two and preserve in Miiller's 

 fluid. 



Samples of the most important tissues have now been obtained. 

 Those preserved in alcohol will probably need no further care: it 

 might be well, however, to look them over occasionally. If, on shak- 

 ing the bottle, little fluft'y pieces fill the liquid, or if a cloudy 

 sediment collect in the bottom, the alcohol should be changed and a 

 few of the specimens removed to another jar. The proportion of 

 hardening fluid to tissue should be about as 10 to 1. If this pro- 

 portion is maintained, trouble will very seldom arise. 



After four- or five days the jars containing Miiller's fluid should 

 be emptied and fresh fluid added; a week later, remove to alcohol aU 

 the specimens hitherto in Miller's fluid, excepting, however, the 

 brain, eye and bone. The work is now over and the specimens will 

 harden nicely without further care. 



