The Microscope. 83 



We notice that Dr. F. L. James, of St. Louis, is soon to publish 

 his excellent articles on microscopical technology in book form. 

 These papers, extracts from which have frequently appeared in 

 The Microscope, were contributed during a period of more than a 

 year to the St. Louis Medical and Surgical Journal, of which Dr. 

 James is editor, and have found a host of readers, who will now be 

 glad to possess them in more convenient, compact and permanent 

 shape. A review of this work will soon be published in our columns. 



Acknowledgments. — We have received from Prof. A. Y. Moore, 

 Cleveland, Ohio, one of his finely stained and mounted slides of 

 urinary casts; from Prof. George R. Koenig, Philadelphia, Pa., 

 diatomaceous earth (Gray's Ferry Road); from Mr. K. M. Cunning- 

 ham, Mobile, Ala., some cleaned Pensacola Bay diatoms, the beauty 

 of which is beyond description; from R. Munger, M. D., San An- 

 tonio, Texas, some photo- micrographic negatives of chicken lice, 

 urinary sediment, foreign bodies in mucus fi'om nose, etc. These 

 negatives were taken with a landscape camera, by lamp light, and 

 the doctor is to be congratulated on the excellence of the results, 

 which indicate long experience in microscopical methods; fi-om Dr. 

 F. P. Peck, of the Iowa Hospital for the Insane, comes a fine photo- 

 micrograph of Koch's comma bacillus ; from Mr. Arthur J. Doherty, 

 of the College Microscopical Depot, Manchester, England, a box of 

 sample slides. We can truthfully say that we have never seen better 

 examples of staining and mounting than as here presented. Micros- 

 copists who wish to fill empty spaces in their cabinet, will do well 

 to consult IVIr. Doherty's catalogue. 



TECHNOLOGY, 



AN IMPROVED METHOD OF PREPARING AND STAINING 

 THE BACILLUS TUBERCULOSIS. 



Henry L. Tohnan in the Medical Record, October 23, gives 

 a modification of the Weigert-Ehrlich method of staining the tuber- 

 cle-bacillus which we have found to be very satisfactory. He uses 

 the following formulse: 



1. Aniline oil M. xxx; distilled water, 3 iij. Shake vigorously 

 for five minutes and filter. 



2. Saturated solution of fuchsine in 93 per cent, alcohol. Pre- 

 pare the sputum in the usual manner by spreading thinly on a cover 

 glass and drying. Pass the glass to and fro in the flame of an alco- 

 hol lamp for fifteen seconds. For staining take of No. 1, 3 ij, and of 

 No. 2, M. XV, and mix. Drop the cover glasses on the liquid con- 



