^2 The Microscope. 



These three conditions have been fulfilled. The germ of 

 the Southern cattle plague has been found in the blood, the 

 gall, the urine, the liver, spleen and kidneys of every animal that 

 we have made an autopsy on that was diseased. These germs have 

 also been cultivated in an absolutely pure form upon and in artificial 

 media. Gophers, or ground squirrels, have been inoculated with 

 such cultivations and died from the effects, and the same germ found 

 in their blood and tissues and in sections made from their organs. 

 Cultivations from the same have also been made, invariably showing 

 the same germ as that got from the cattle. 



These results, however, do not show that this was the germ of 

 Southern cattle plague. They only show that a germ was found in 

 the tissues of diseased animals that had fatal disease producing 

 properties. 



How, then, can we tell that it is the specific germ of the 

 Southern cattle plague? 



To be able to affirm this fact positively cattle must be inocu- • 

 lated, as the ground squirrels were, with unquestionably pure culti- 

 vations, and the Southern cattle plague produced in those cattle, and 

 the same germ found in their tissues and cultivated from them. "We 

 have done this and can demonstrate the entire series of facts by cult- 

 ures and microscopic specimens of the tissiies. 



Hence the germ of the Southern cattle plague has been found, 

 and I think I may be pardoned in claiming this to he the first occa- 

 sion in the history of American medicine that not only one, but two 

 germ-diseases of animal life have been thoroughly traced out and 

 placed upon an impiregnable scientific basis. 



The sun of original investigation seems to be rising in the west 

 so far as America is concerned. 



This honor belongs to Nebraska, and the credit of it is equally 

 to be shared by the State board of agriculture that suggested the 

 work, and the board of regents of the State University who have 

 made the work possible. I challenge the world to show more credit- 

 able work done by the most noted European investigators within the 

 period of fourteen months in the history of medicine, without more 

 than alluding to the many disadvantages under which the work had 

 to be done for want of proper resources, material and means, espe- 

 cially up to the present time, in comparison to the advantages which 

 the European governments offer their workers. These remarks are 

 not offered in complaint. Such stations as this are gradual growths. 



