278 The Microscope. 



Seamen, after which President Lewis declared the eleventh annual 

 meeting of the American Society of Microscopists adjourned sine die. 

 We regret that our limited space will not permit us to give a 

 more detailed account of the proceedings of the Columbus meeting. 

 We can only say that the whole affair was most enjoyable and profit- 

 able; that the papers read were of a high order and of great scien- 

 tific value; that the bond of good fellowship was more closely knit- 

 ted, and that all present left for their respective homes determined 

 that the meeting for the coming year should be the best ever held 

 by the Society. Buffalo, N. Y., will probably be selected as the 

 most desirable point for the next annual session. 



ELEMENTARY DEPARTMENT. 



RUDIMENTS OF PRACTICAL EMBRYOLOGY.* 



W. p. MANTON. 

 THRELF all's METHOD. 



§ 12. In this method a film of India-rubber is used on the 

 slide instead of shellac. The India-rubber solution is made by 

 dissolving the pure gum, which may be had in any quantity of 

 dealers in rubber goods, in chloroform or benzine. 



The rubber must be cut up into very small pieces and placed 

 in the solvent. But little of the former is necessary, and it 

 dissolves quite slowly. 



A film of the rubber solution is spread on the slide by means 

 of a brush, and allowed to dry. The sections are arranged on the 

 slide, which is then heated as above directed, allowed to cool, and 

 then placed in benzolinef for a few moments, to dissolve out the 

 paraffin, and then mounted in balsam. 



If desired, the sections may be stained after they have been 

 fixed to the slide. To do this, after immersing in the benzo- 

 line, wash in absolute alcohol, then in weaker alcohol, stain, 

 wash in 95 per cent, alcohol, return to absolute alcohol, clear with 

 clove oil, and mount in balsam. Creasote may be substituted for 

 the clove oil, but the latter is better. 



The disadvantage of this method is that the sections remain 

 loose until fixed by the heat, and are therefore liable to become 

 disarransred and scattered. 



* Copyrighted 1888. 



tXylol or any other solvent may be used. 



