The Microscope. 279 



Dissolve the gun cotton in the ether and alcohol, add the clove 

 oil, and filter. 



Label — Schallibaum's Clove-oil Collodion. 



A thin layer of this collodion should be painted on the slide 

 just before using. 



When the sections have been arranged, heat the slide carefully 

 over the spirit-lamp flame, let cool, and immerse in some solvent, as 

 benzine, xylol, chloroform, turpentine, etc., to get rid of the 

 paraffin. Rinse in 95 per cent, alcohol and clear in the following 

 agent: — 



i^ Turpentine, 3 parts. 



Carbolic acid, 2 parts. 



Mix. 

 Label — Clearing mixture. 



The sections are then protected by a cover-glass which has 

 received a thin coating of the following: — 



I^ Pure Canada balsam, 25 gms. 



Chloroform, 2 cc. 



Clove oil, 2 cc. 



Mix. 

 The clove oil dispels all cloudiness. 



If the specimen has not been stained in toto, the sections on 

 the slide may be treated in this manner: After immersing in the 

 solvent and rinsing in 95 per cent, alcohol, place in the stain imme- 

 diately, if this is an alcoholic solution, otherwise wash first with 

 water. On removing from the stain wash in alcohol, or first in 

 water if the stain is an aqueous solution. Clear and mount as 

 above. 



The Microscopical Bulletin calls attention to the fact that in 

 determining the magnifying power of a lens, the rule used should 

 always be placed at a uniform distance of ten inches from the eye, 

 and not on the stage of the microscope, unless that happens to be at 

 the required distance, 



♦Proceedings Am. Society of Microscopists, 1884. 



