The Microscope. 333 



at each membrane of Krause, and longitudinal connecting rods. The 

 network, like the sarcolemma, seems to resist the action of the acetic 

 acid more than the matrix or sarcous substance. If the fiber be 

 stained in haematoxylin after the action of the acetic acid, the net- 

 work becomes stained to a greater extent than the matrix. 



Osmic acid preparations are made by placing living muscles 

 from the bee in the acid solution (i per cent.), for 10 minutes; 

 mount in Farrant's medium or balsam, and I would recommend the 

 balsam made by the Palmer Slide Co. 



Living fiber : The chitinous integument of the leg of the bee or 

 dytiscus; slit longitudinally, scoop the muscle out, and quickly tease 

 on a cover-glass and invert over the moist gas chamber. 



Alcohol preparations : 



I find that spirit has a tendency to split the fiber into fibrils 

 and sarcous elements. After the muscle has been in alcohol it may 

 be stained with some reagents. Kleinberg's haematoxylin, for 

 instance, gives excellent results. Alum carmine may also be used. 

 Mount in Canada balsam. 



There is an excellent article in " Studies from the Biological 

 Laboratory of Owen's College, England," by Dr. B. Melland. 



BIBLIOGRAPHY. 



Quart. Jour, of Micros. Sc, 1886. J. R. M. Soc, 1886. And the 

 various works on Histology. 



PROCEEDINGS OF SOCIETIES 



ILLINOIS STATE MICROSCOPICAL SOCIETY. 



A MEETING of this society was held at the residence of Prof. 

 -*■ ^ M. D. Ewell, South Evanston, 111., on Friday evening, Octo- 

 ber 11th. Prof. Ewell exhibited one large Rogers automatic divid- 

 ing engine. This engine is the one a full description of which was 

 published by The Miceoscope ; one small dividing engine, with screw 

 three inches long; one large Rogers comparator, 114 centimeters 

 long; one small Rogers comparator, four inches long; a variety of 

 standards from full yard and meter down to one centimeter; a set 

 of Kew standard thermometers; two standards by Bauchier, Paris; 

 a set of Geissler thermometers, and two exceptionally fine standards 

 by N. J. Green, of New York. These last are practically perfect. 

 Also, boiling point coefficient of expansion apparatus, etc. The 



