340 The Microscope. 



8. Robin's solution is a saturated solution of 



sulphate of sodium. 



9. Richardson's salt solution. 



Chloride of sodium 0.75 parts. 



Water 100. 



Having the corpuscles isolated by this 



liquid, he stains them with a little 



anilin. 



10. Welcker's fluid. 



Glycerine 1 part. 



Water 7 " 



11. He also uses the following solution (artificial 



serum). 



Chloride of sodium 4 part's. 



Egg albumen 300 " 



Water 2700 " 



12. Malinin' 8 solution. Saturated alcoholic solution 



of caustic potash. (90 per ct. alcohol.) 

 Dr. Formad proceeds to examine a specimen in this way: A 

 small granule of the suspected blood, or a fiber from the blood- 

 stained fabric is placed on a glass slide in a drop of a 30 to 35 per 

 cent, solution of caustic potash, and covered with a cover-glass. If 

 the blood stain was recent, the disintegration of the clot commences 

 at once, and the isolated corpuscles separate and swim swiftly 

 through the liquid, if the stage of the microscope is slightly inclined. 

 * * * In direct proportion to the age of the stain, from one to 

 within ten days, the softening of the microscopic blood mass and the 

 isolation of the corpuscles is protracted. In blood older than ten 

 days, the ratio of softening or disintegration cannot be well observed, 

 an d a stain of two years old behaved like one of ten days. 



Serial Sections with Celloidin.* — The celloidin block, with 

 the object imbedded, is cut as regularly as possible, and fastened to 

 a cork. In sectioning, the knife should be placed nearly parallel 

 with its direction of motion, and after every five to ten sections wet 

 with 95 per cent, alcohol. The sections are raised from the knife 

 with a small brush, and placed on the surface of bergamot oil (in a 

 small glass dish over a white ground). If the oil is good the sec- 

 tions will at once unroll and become transparent. 



♦ J Apathv. MetliodB zur Verf^Tfipuns lan?er<-i Scliiiittsprien niit Celloidin. Mitth. 

 a. d. zool. Station z. Neapel., vii , 4, p. 742, ISbT.—Am. Naturalist. 



