210 The Microscope. 



gree without detriment. This is my plea for the short slide. Will 

 the reader allow me to submit it for its worth, without expecting 

 to see its general adoption, but hoping to help someone who, 

 like myself, is often bothered about the close working oljjectives, 

 that in spite ©f all carefuUness, occasionally impinge up®n the 

 cover glass. Racking downward is very troublesome to old eyes 

 using high powers. They find it difficult to look across the 

 stage to reach below the focus, where the looking distance is one 

 one-hundredth inch or less. Let all such try my safety slide, 

 and they will find its perfect working convenieiice an ample 

 compensation for being out of fashion or for lack of imaginary 

 beauty, and after using it often and long, and trying it faithfully 

 and well, they will, like myself, never wish to see another long 

 slide for ordinary mici'oscopical work. 



REPEllENCE TABLES FOR MICROSCOPICAL WORK. 



IV. — ANILINE STAIN.S. 

 COMI'ILED BY PROFESSOR A. B. AUBERT. 



A LUM EOSIN : Eosin, 1 part; alum, I part; alcohol, 200 parts. 

 i*- Reagent for haemoglobin. Specimens previously treated 

 with osmic acid ^ per cent for 3 minutes. Wash thoroughly 

 before stair. ing. 



Aniline blue, (water solution): Aniline blue, 0.02 grras.; water, 

 25 c. c; alcohol, 25 to oO drops. Specimens hardened in alcohol. 



Aniline black: Aniline black, 0.5 grras.; alcohol, 99 c. c; water, 

 1 to 2 c. c. Stains in a few minutes; for brain, etc. 



Bismarck brown: 1. Concentrated aqueous solution, warm, or 

 weak alcoholic solution. 2. Bismarck brown, 1 part; water, 3,000 

 to 5,000 parts. For protoplasm, connective tissue, bacteria, liv- 

 ing organisms, etc. Material to be hardened in alcohol or chro- 

 mic acid ; wash in absolute alcohol. Mount in glj-cerine or 

 balsam. 



Borax methylin blue: Concentrated aqueous solution of blue, 

 24 vols.; borax solution, 5 per cent, 16 vols.; water, 40 vols. 

 Dissolve ; filter after 24 hours. • 



Chinolin blue: Aqueous solution, 1-100,000 to 1-500,000. For 

 living organisms (water analysis), etc. 



