The Microscope. 271 



Dahlia or iioffman's violet: Glacial acetic acid, 12.5 c. c. ; 

 absolute alcohol, 50 c. c. ; water, 100 c. c. ; dahlia nearly to satu- 

 ration. For axis cylinder of nerves, protoplasm, nucleus. Stains 

 in 12 hours or less. 



EosiN : Water solution, or water solution and one-third of 

 alcohol; or eosin, 1 part, and water 1,000 to 1,500 parts. For 

 epithelium, muscle, axis cylinder, amjdoid degeneration, nu- 

 cleus, etc. Stains in ^ to 1 minute or more. 



Gentian violet : Filtered 3 per cent, aniline solution in 

 water ; concentrated gentian violet solution in alcohol ; or gen- 

 tian violet, 2.00 ; ammonia, 0.5 ; absolute alcohol, 10. For bac- 

 teria, etc. At ordinaiy temperature stains in about 24 hours ; 

 1 hour at 50° C. Treat objects with 30 per cent, hydrochloric 

 acid, dehydrate in absolute alcohol, clear in oil of cloves, mount 

 in balsam. 



Iodine ureen : Iodine green, 0.1 part; water or alcohol, 35 

 parts. Stains in a moment. Mount in balsam. 



FucHSiN (rosanilin) : Fuchsin, 0.25 grms. ; alcohol, 20 c. c. ; 

 water, 20 c. c. For nucleus, protoplasm, axis cylinder, elastic 

 tissue, retina, etc. ; after staining, treat with alcohol. 



Fuchsin (acid): Concentrated solution in water. For nervous 

 system. Sections hardened in chromic salts. Keep in stain one 

 hour, wash with water, put into alcoholic solution of potash 

 (potash, 1 grm.; alcohol, 100 c. c; filter after twenty -four hours ; 

 use 10 c. c. diluted with 100 c. c. of alcohol); wash in water, 

 dehydrate in alcohol (saturated with salt), mount in balsam. 



Metuylin blue : 1. Frlich's concentrated water solutien. 2 

 Koch's concentrated alcoholic solution : Methyl blue, 10 ; caustic 

 potash (10 per cent. 0.2; water 200). 1 and 2 for bacteria, 

 cover glass preparations ; stains in one-half to twenty-four 

 hours ; wash in water, dry, mount in balsam. For tubercle 

 bacillus ; after staining in blue, transfer cover to concentrated 

 solution of vesuvin (15 minutes), wash well in water, dehydrate 

 in alcohol, clear in oil of cloves (micrococcus brown ; bacillus 

 blue). 



Methyl violet : Methyl violet concentrated alcoholic solu- 

 tion, 11 c. c. ; absolute alcohol, 10 c. c. ; aniline water, 100 c. c. 

 For bacteria, etc. ; cover glass preparations ; stains in 24 hours ; 

 put cover for a few seconds in nitric acid and 3 parts water ; 

 wash with alcohol ; stain with diluted vesuvin solution for a 



