ie ge SEAN. 45 
discoloured surface of the wood could be seen. In the other two plants the fungus 
spread up and down one side of the stem but did not succeed in ringing it, and 
these plants retained their leaves as long as their healthy neighbours. 
Experiment III. Three plants were infected with pure cultures of D. Corchori, 
each plant being wounded by means of a tangential cut on the stem surface. 
These wound infections were all left exposed to the air. Two of the plants died 
within 10 days, the other was not completely rmged by the disease and survived 
longer. 
As the season was now far advanced and the crop was drying off, further inocu- 
lation work was stopped. These preliminary experiments had, however, shown 
that the fungus was capable of infecting both unwounded and wounded 
healthy jute stems. Inoculation experiments upon green-stemmed C. capsularis 
were resumed in 1918. - 
Experiment IV. Two pots were sown with jute on 12th March, 1918, and 
four young plants were infected from an agar culture of D. Corchori on 22nd April, 
1918. The pot was kept under a large hell jar ; another pot sown at the same time, 
but not infected, was also kept under a bell jar. The plants in both infected and 
control pots lost their leaves, from being kept under a bell jar, im fivedays. The 
infections did not take. 
Experiment V. Five plants, sown in a pot on 12th March, 1918, were infected 
with a young, 48 hour old, agar culture of D. Corchori on 2nd May, 1918. Four 
of the plants were infected at a leaf base and one on the stem. All the infect- 
ed plants, and an equal number of uninfected plants were kept under bell jars. 
All the infected leaves wilted and fell off by 7th May, 1918, and in one case a black 
stain commenced to spread from an infected leaf base up the stem and a micros- 
copic examination showed that Diplodia hyphe were present. No stem damage 
was observed in the other case, and by 13th May, 1918, the plants, both infected 
and control, had become unhealthy from being kept under a bell jar. No definite 
conclusion as to whether the fungus could infect young jute stems could be drawn 
from these last two experiments. 
Experiment VI. Twoplants sown in a pot on 12th March, 1918, were mfected 
from an agar culture, 48 hours old, of D. Corchori on 27th May, 1918. The length 
of stem within which the infection was done was enclosed in a glass lamp chimney, 
the ends of which were plugged with cotton wool. After 24 hours a brown stain 
was distinctly visible at the seat of each infection, and by 3lst May the discolora- 
tion had spread and one plant was nearly ringed ; in both plants the leaves were yel- 
lowing and falling. Both plants were ringed by a black band by 3rd June, and 
Diplodia pycnidia and spores were clearly visible on the surface of the diseased 
tissues, One of the plants was completely wilted and dead by 6th June, and an 
